|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to residues C(15) H L D I I W(21) of endothelin.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-020 detects endothelin (ET)-1, ET-2 and ET-3. This antibody shows no cross-reactivity with the peptides ET(15-20) and ET(15-22).
MA3-020 has been successfully used in ELISA procedures. By ELISA this antibody is useful in solid-phase or competitive solution based procedures particularly in the study of enzymatic processing of ET, and in the identification of putative enzyme inhibitors. MA3-020 can detect approximately 0.5 pmol/ml ET-1 by direct ELISA.
The MA3-020 immunogen is a synthetic peptide corresponding to residues C(15) H L D I I W(21) of endothelin. This sequence is conserved between numerous species.
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms- ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Carbohydrate metabolism Signal transduction Cell surface receptor mediated signal transduction G-protein mediated signaling Cell communication Ligand-mediated signaling Transport Carbohydrate transport Homeostasis Blood circulation and gas exchange Regulation of vasoconstriction, dilation Cell proliferation and differentiation