|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COOH-terminal amino acids of mouse ETA (408-427) KNQEQNNHNTERSSHKDSMN coupled to KLH|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:2000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-065 detects Endothelin Receptor A from human, mouse, and rat samples.
PA3-065 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By western blot, this antibody detects a 46kDa protein corresponding to mouse Endothelin Receptor A.
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8.
PA3-065 was used in immunohistochemistry - paraffin section to characterize a monoclonal rabbit anti-ETA antibody UMB-8 in and test in sarcomas and in glioblastomas
|Lupp A,Mann A,Heeb A,Kaemmerer D,Sänger J,Evert M,Evert K,Mawrin C,Schulz S||Peptides (66:19)||2015|
Transforming growth factor-ß regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.
PA3-065 was used in western blot to investigate the influence of tumor growth factor beta on endothelin-1 expression and effects of hypoxia on the newborn lung
|Olave N,Nicola T,Zhang W,Bulger A,James M,Oparil S,Chen YF,Ambalavanan N||American journal of physiology. Lung cellular and molecular physiology (302:L857)||2012|