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|Tested species reactivity||Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (NHSTTAKEGKSDSE) corresponding to the C-terminus amino acids of EN2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-0.3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine, human and mouse based on sequence homology.
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AUTS1; EN2; engrailed 2; engrailed homolog 2; Engrailed-2; homeobox protein engrailed-2