|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Partially purified preparation of laminin from the EHS mouse tumor.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
MA1-06501 detects entactin in human samples.
MA1-06501 has sucessfully been used in Western blotting, immunosytochemistry and immunohistochemistry. By Western blotting, it detects a ~150 kDa protein representing entactin.
The MA1-06501 immunogen is partially purified preparation of laminin from the EHS mouse tumor.
Store at 4°C or in small aliquots at -20°C.
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen,laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulphated glycoprotein of approximately ~150 kD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Distinct spatio-temporal extracellular matrix accumulation within demyelinated spinal cord lesions in Theiler's murine encephalomyelitis.
MA1-06501 was used in immunohistochemistry to study the distinct matrix accumulation within demyelinated spinal cord lesions in Theiler's murine encephalomyelitis
|Haist V,Ulrich R,Kalkuhl A,Deschl U,Baumgärtner W||Brain pathology (Zurich, Switzerland) (22:188)||2012|
|Not Applicable||Not Cited||
Foxf1 and Foxf2 control murine gut development by limiting mesenchymal Wnt signaling and promoting extracellular matrix production.
MA1-06501 was used in immunohistochemistry to elucidate the roles of Foxf1 and Foxf2 in vertebrate gut development
|Ormestad M,Astorga J,Landgren H,Wang T,Johansson BR,Miura N,Carlsson P||Development (Cambridge, England) (133:833)||2006|
Uterine extracellular matrix components are altered during defective decidualization in interleukin-11 receptor alpha deficient mice.
MA1-06501 was used in immunohistochemistry to investigate the effect of IL-11 on the uterine extracellular matrix
|White CA,Robb L,Salamonsen LA||Reproductive biology and endocrinology : RB and E (2:null)||2004|