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Enterokinase Antibody (A00889-40) in WB
Western blot analysis of Enterokinase protein using Enterokinase Antibody, mAb, Mouse (GenScript, A00889, 1 µg/mL). The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensor TM HRP Substrate Kit (GenScript, L00221). Predicted Size: 38 kD. Observed Size: 38 kD.
Product Details
A00889-40
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5E3C8
Immunogen
Purified recombinant light chain of bovine EK protein
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Enterokinase is an intestinal enzyme responsible for initiating activation of pancreatic proteolytic proenzymes. Mouse Anti-Enterokinase Monoclonal Antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant EK protein (bovine EK, aa848-1035, light chain, 93% homologous with human EK).Mouse Anti-Enterokinase Monoclonal Antibody recognizes EK in Western blots and ELISAs.
Target Information
This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Enterokinase; Enterokinase light chain; Enteropeptidase; enteropeptidase, light chain (L chain); protease, serine, 7 (enterokinase); Serine protease 7; Transmembrane protease serine 15; transmembrane protease, serine 15
Gene Aliases: ENTK; PRSS7; TMPRSS15
UniProt ID: (Bovine) P98072, (Pig) P98074
Entrez Gene ID: (Bovine) 282009, (Pig) 397152
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