|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant human eotaxin 2, expressed in E. coli.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||2 µg/ml|
|Inhibition Assays (IA)||1-5 ug/ml|
|Neutralization (Neu)||1-5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24653 detects Eotaxin 2 from human samples.
MA1-24653 has been successfully used in inhibition and neutralization assays, and Western blot procedures. The Neutralization Dose50 (ND50) for this antibody is approximately 1 to 5 ug/ml in the presence of 100 ng/ml of recombinant human eotaxin 2, measuring the chemotaxis of rat myeloid Y3 cells transfected with the human CCR3 gene.
The MA1-24653 immunogen is purified, recombinant human eotaxin 2, expressed in E. coli.
Eotaxin is a 74-amino acid, eosinophil-chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.