|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length mouse protein.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5 µg/ml|
|Functional Assay (FN)||7-8 ug/ml|
|Inhibition Assays (IA)||7-8 ug/ml|
|Neutralization (Neu)||7-8 µg/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25517 detects Eotaxin 2 from mouse samples.
PA1-25517 has been successfully used in inhibition assays, functional assays, neutralization and Western blot procedures. For neutralization, use at a concentration of 7-8 ug/ml to achieve half of the neutralizing dose (ND50) of a 100.0 ng/ml concentration.
The PA1-25517 immunogen is recombinant, full length mouse protein.
Eotaxin is a 74-amino acid, eosinophil-chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.