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Immunofluorescence analysis of EpCAM / CD326 was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of EpCAM / CD326 (Ber-EP4) Mouse Monoclonal Antibody (MA512604) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||MCF-7 human breast carcinoma cell line|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-12604 targets Epithelial Specific Antigen in IHC (P) applications and shows reactivity with Human samples.
The MA5-12604 immunogen is mCF-7 human breast carcinoma cell line.
Epithelial Specific Antigen / Ep-CAM
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Primary localized malignant biphasic mesothelioma of the liver in a patient with asbestosis.
MA5-12604 was used in immunohistochemistry to report a clinical case of primary localized malignant biphasic mesothelioma of the liver associated with asbestosis
|Sasaki M,Araki I,Yasui T,Kinoshita M,Itatsu K,Nojima T,Nakanuma Y||World journal of gastroenterology (15:615)||2009|
adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP40; EPCAM; epithelial glycoprotein 314; Epithelial Specific Antigen; ESA; human epithelial glycoprotein-2; KS1/4; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; tumor-associated calcium signal transducer 1
DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1