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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Native Ep-CAM from lung tissue.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:100-1:500|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06502 detects Ep-CAM in human samples.
MA1-06502 has sucessfully been used in Western blotting, immunocytochemistry and immunohistochemistry. By Western blotting, it detects a ~40 kDa protein representing Ep-CAM. Western blot should be performed under non-reducing conditions.
The MA1-06502 immunogen is native Ep-CAM from lung tissue. This antibody reacts with the first EGF repeat in the extracellular domein of Ep-CAM. The epitope is sensitive for reducing agents.
Store at 4°C, or in small aliquots at -20°C.
Ep-CAM is a ~40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
New frontiers in circulating tumor cell analysis: A reference guide for biomolecular profiling toward translational clinical use.
MA1-06502 was used in immunocytochemistry to review the current state-of-the-art in circulating tumor cell profiling
|Becker TM,Caixeiro NJ,Lim SH,Tognela A,Kienzle N,Scott KF,Spring KJ,de Souza P||International journal of cancer (134:2523)||2014|
Neuropilin-2 Is upregulated in lung cancer cells during TGF-β1-induced epithelial-mesenchymal transition.
MA1-06502 was used in western blot to study TGF-beta1-driven lung cancer cell epithelial-to-mesenchymal transition and the role played by neuropilin-2
|Nasarre P,Gemmill RM,Potiron VA,Roche J,Lu X,Barón AE,Korch C,Garrett-Mayer E,Lagana A,Howe PH,Drabkin HA||Cancer research (73:7111)||2013|
Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells.
MA1-06502 was used in western blot to study the role of ZEB1 in mediating the growth of malignant pleural mesothelioma cells
|Horio M,Sato M,Takeyama Y,Elshazley M,Yamashita R,Hase T,Yoshida K,Usami N,Yokoi K,Sekido Y,Kondo M,Toyokuni S,Gazdar AF,Minna JD,Hasegawa Y||Annals of surgical oncology (19 Suppl 3:S634)||2012|
Systematic analysis and validation of differential gene expression in ovarian serous adenocarcinomas and normal ovary.
MA1-06502 was used in immunohistochemistry to identify genes differentially expressed in ovaraian serous adenocarcinoma as compared to healthy ovarian tissue
|Bauerschlag D,Bräutigam K,Moll R,Sehouli J,Mustea A,Salehin D,Krajewska M,Reed JC,Maass N,Hampton GM,Meinhold-Heerlein I||Journal of cancer research and clinical oncology (139:347)||2013|
Differential gene expression in normal esophagus and Barrett's esophagus.
MA1-06502 was used in immunohistochemistry to investigate the change of gene expression patterns in Barrett esophagus
|Wang J,Qin R,Ma Y,Wu H,Peters H,Tyska M,Shaheen NJ,Chen X||Journal of gastroenterology (44:897)||2009|
Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.
MA1-06502 was used in immunohistochemistry to investigate Lynch syndrome with MSH2-deficient tumors
|Ligtenberg MJ,Kuiper RP,Chan TL,Goossens M,Hebeda KM,Voorendt M,Lee TY,Bodmer D,Hoenselaar E,Hendriks-Cornelissen SJ,Tsui WY,Kong CK,Brunner HG,van Kessel AG,Yuen ST,van Krieken JH,Leung SY,Hoogerbrugge N||Nature genetics (41:112)||2009|
Gene expression patterns in ovarian carcinomas.
MA1-06502 was used in immunohistochemistry to study gene expression patterns in ovarian carcinomas using DNA microarrays
|Schaner ME,Ross DT,Ciaravino G,Sorlie T,Troyanskaya O,Diehn M,Wang YC,Duran GE,Sikic TL,Caldeira S,Skomedal H,Tu IP,Hernandez-Boussard T,Johnson SW,O'Dwyer PJ,Fero MJ,Kristensen GB,Borresen-Dale AL,Hastie T,Tibshirani R,van de Rijn M,Teng NN,Longacre TA,Botstein D,Brown PO,Sikic BI||Molecular biology of the cell (14:4376)||2003|
Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.
MA1-06502 was used in flow cytometry to study the different molecular phenotype and tumorigenicity of 5 breast cancer cell lines derived from a single patient
|Mosoyan G,Nagi C,Marukian S,Teixeira A,Simonian A,Resnick-Silverman L,DiFeo A,Johnston D,Reynolds SR,Roses DF,Mosoian A||PloS one (8:null)||2013|
adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP40; EPCAM; epithelial glycoprotein 314; Epithelial Specific Antigen; ESA; human epithelial glycoprotein-2; KS1/4; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; tumor-associated calcium signal transducer 1
DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1