Western blot analysis of Eph receptor A4 using A) 30 µg 293T whole cell extract and B) 5 µg whole cell extract of 3xFlag-human EPHA4-transfected 293T cells. Samples were loaded onto a 5% SDS-PAGE gel and probed with an Eph receptor A4 polyclonal antibody (Product # PA5-27998) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 350 and 697 of Human Eph receptor A4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27998 targets Eph receptor A4 in WB applications and shows reactivity with Human samples.
The PA5-27998 immunogen is recombinant fragment corresponding to a region within amino acids 350 and 697 of Human Eph receptor A4.
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.