Invitrogen

EphA2 Monoclonal Antibody (1C11A12)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

12 References

View all (35) EphA2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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EphA2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of EphA2 was performed using 70% confluent log phase A549 cells treated with UV-302 nm for 60 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EphA2 (1C11A12) Mouse Monoclonal Antibody (Product # 37-4400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

EphA2 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A-431 (Lane 1), A-431+UV release (5 hour treatment with UVR, after treatment release for 12 hours) (Lane 2) and PC-3 (Lane 3). The blots were probed with Anti-EphA2 Receptor Mouse Monoclonal Antibody (Product # 37-4400, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 108 kDa and 54 kDa corresponding to EphA2 was observed in A431 upon UV treatment and release and one isoform of 108 kDa was observed in PC-3. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

EphA2 Antibody in Immunohistochemistry (IHC) — Figure 5 Concomitant Ephrin B3, Ephrin A1 and EphA2 expression in NSCLC clinical specimens Ephrin B3, Ephrin A1 and EphA2 expression levels and their relation to patient overall survival (OS) was analyzed in a cohort of Stage IA-Stage IB NSCLC specimen using immunohistochemistry. A. Examples of Ephrin B3, EphA2 and Ephrin A1 staining in adenocarcinoma and squamous cell carcinoma NSCLC specimens. 20x magnification. B. Kaplan-Maier curves showing the association between Ephrin B3, EphA2 and Ephrin A1 expression intensity levels and OS in the NSCLC patient cohort. Red line: Low score, black line: High score. Wilcoxon test was used for statistical assessment and the p-values are indicated.

EphA2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of EphA2 using EphA2 monoclonal antibody (Product # 37-4400), shows induction of EphA2 expression in the cytoplasm of A549 cells upon UV treatment. {TM}

Product Details

37-4400

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	2 µg/mL	View 2 publications 2 publications
ELISA (ELISA)	Assay-dependent	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	1C11A12
Immunogen	Synthetic peptide derived from the C-terminal region of the human EphA2 Receptor protein. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533318

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EPHA2 Interacts with DNA-PK_cs in Cell Nucleus and Controls Ionizing Radiation Responses in Non-Small Cell Lung Cancer Cells.

Cancers

Kaminskyy VO,Hååg P,Novak M,Végvári Á,Arapi V,Lewensohn R,Viktorsson K

37-4400 was used in Western Blot to elucidate the function of the Ephrin type-A receptor 2 on the DNA damage response pathway involved in resistance to ionizing radiation.

Sun Feb 28 00:00:00 EST 2021

microRNA-451a promoter methylation regulated by DNMT3B expedites bladder cancer development via the EPHA2/PI3K/AKT axis.

BMC cancer

Liu B,Sun W,Gao W,Li L,Cao Z,Yang X,Liu J,Guo Y

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Western Blot

Wed Oct 21 00:00:00 EDT 2020

A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax.

Malaria journal

Pewkliang Y,Rungin S,Lerdpanyangam K,Duangmanee A,Kanjanasirirat P,Suthivanich P,Sa-Ngiamsuntorn K,Borwornpinyo S,Sattabongkot J,Patrapuvich R,Hongeng S

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Immunofluorescence

Thu Jan 25 00:00:00 EST 2018

Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis.

Nature communications

Lee H,Noh H,Mun J,Gu C,Sever S,Park S

37-4400 was used in immunocytochemistry describe the role of Anks1a in COPII-mediated EphA2 export from the endoplasmic reticulum

Tue Sep 13 00:00:00 EDT 2016

Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis.

Nature communications

Lee H,Noh H,Mun J,Gu C,Sever S,Park S

37-4400 was used in immunocytochemistry describe the role of Anks1a in COPII-mediated EphA2 export from the endoplasmic reticulum

Tue Sep 13 00:00:00 EDT 2016

Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer.

Oncotarget

Efazat G,Novak M,Kaminskyy VO,De Petris L,Kanter L,Juntti T,Bergman P,Zhivotovsky B,Lewensohn R,Hååg P,Viktorsson K

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Miscellaneous

Tue Sep 13 00:00:00 EDT 2016

Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer.

Oncotarget

Efazat G,Novak M,Kaminskyy VO,De Petris L,Kanter L,Juntti T,Bergman P,Zhivotovsky B,Lewensohn R,Hååg P,Viktorsson K

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Miscellaneous

Tue Sep 13 00:00:00 EDT 2016

Characterization of a novel angiogenic model based on stable, fluorescently labelled endothelial cell lines amenable to scale-up for high content screening.

Biology of the cell

Prigozhina NL,Heisel A,Wei K,Noberini R,Hunter EA,Calzolari D,Seldeen JR,Pasquale EB,Ruiz-Lozano P,Mercola M,Price JH

Sat Oct 01 00:00:00 EDT 2011

EphA2 immunoconjugate as molecularly targeted chemotherapy for ovarian carcinoma.

Journal of the National Cancer Institute

Lee JW,Han HD,Shahzad MM,Kim SW,Mangala LS,Nick AM,Lu C,Langley RR,Schmandt R,Kim HS,Mao S,Gooya J,Fazenbaker C,Jackson D,Tice DA,Landen CN,Coleman RL,Sood AK

37-4400 was used in immunohistochemistry and western blot to test if an anti-EphA2 monoclonal antibody linked to a chemotherapeutic agent has antitumor activity using ovarian cancer cell lines and tumor models

Wed Sep 02 00:00:00 EDT 2009

EphA2 immunoconjugate as molecularly targeted chemotherapy for ovarian carcinoma.

Journal of the National Cancer Institute

Lee JW,Han HD,Shahzad MM,Kim SW,Mangala LS,Nick AM,Lu C,Langley RR,Schmandt R,Kim HS,Mao S,Gooya J,Fazenbaker C,Jackson D,Tice DA,Landen CN,Coleman RL,Sood AK

Wed Sep 02 00:00:00 EDT 2009

A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo.

Cancer research

Jackson D,Gooya J,Mao S,Kinneer K,Xu L,Camara M,Fazenbaker C,Fleming R,Swamynathan S,Meyer D,Senter PD,Gao C,Wu H,Kinch M,Coats S,Kiener PA,Tice DA

Sat Nov 15 00:00:00 EST 2008

Framework shuffling of antibodies to reduce immunogenicity and manipulate functional and biophysical properties.

Molecular immunology

Damschroder MM,Widjaja L,Gill PS,Krasnoperov V,Jiang W,Dall'Acqua WF,Wu H

37-4400 was used in western blot to characterize monoclonal antibodies that recognize EphA2 and EphB4

Sun Apr 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: ephrin receptor EphA2; Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2

Gene Aliases: ARCC2; AW545284; CTPA; CTPP1; CTRCT6; ECK; EPHA2; Myk2; Sek-2; Sek2

UniProt ID: (Human) P29317, (Mouse) Q03145

Entrez Gene ID: (Human) 1969, (Mouse) 13836, (Rat) 366492

Molecular Function: transmembrane signal receptor