View (6) other EphA2 antibodies
EphA2 Monoclonal Antibody (1C11A12)
Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated.
Catalog # 37-4400
(USD) 309.00, 100 µg
Save to list
Product Image Gallery
Immunofluorescent analysis of EphA2 was performed using 70% confluent log phase A549 cells treated with UV-302 nm for 60 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EphA2 (1C11A12) Mouse Monoclonal Antibody (Product # 37-4400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A-431 (Lane 1), A-431+UV release (5 hour treatment with UVR, after treatment release for 12 hours) (Lane 2) and PC-3 (Lane 3). The blots were probed with Anti-EphA2 Receptor Mouse Monoclonal Antibody (Product # 37-4400, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 108 kDa and 54 kDa corresponding to EphA2 was observed in A431 upon UV treatment and release and one isoform of 108 kDa was observed in PC-3. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Western blot analysis of EphA2-transfected 293 cell lysates using Ms anti-EphA2 Receptor (Product # 37-4400).
Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of EphA2 using EphA2 monoclonal antibody (Product # 37-4400), shows induction of EphA2 expression in the cytoplasm of A549 cells upon UV treatment.
Figure 5 Anks1a influences breast tumorigenesis by regulating the ER export of EphA2-ErbB2 complexes. ( a ) Anks1a +/- female mice were crossed with Anks1a +/- ; MMTV-Neu male mice to obtain female mice with the indicated genotypes. All experiments monitoring breast tumour formation were carried out in mice of the FVB genetic background ( n =16, 29, 14 per genotype). ** P <0.01, chi 2 -test. ( b , c ) PMTCs were isolated from breast tumours of MMTV-Neu female mice and transduced with Anks1a -specific shRNA lentiviruses. Infected cells were subjected to soft agar colony formation assays as described in Fig. 4e . Scale bar, 250 mum (top panel); 50 mum (bottom panel). *** P <0.001, Student's t -test. ( d , e ) PMTCs were stained with ephrinA5-Fc or an anti-ErbB2 antibody without permeabilization to detect cell surface receptors as described in Fig. 1c . Scale bar, 20 mum. ** P <0.01, Student's t -test. ( f ) HEK293 cells were co-transfected with the indicated constructs and COPII vesicle-budding assays were performed as described in Fig. 2f ( n =4). Note the use of Sar1a H79G as a control for the normalization of the EphA2 budding. * P <0.05, one-way analysis of variance test. ( g ) Model in which Anks1a facilitates the export of EphA2 and ErbB2 RTKs from the ER. In mitogen-activated cells, Ser-647 and Ser-663 of Anks1a are likely the major regulatory phosphorylation sites. Our experiments indicate that serine phosphorylation of Anks1a is critical for its subcellular localizatio
Published figure using EphA2 monoclonal antibody (Product # 37-4400)
Western Blot (WB)
Host / Isotype
Synthetic peptide derived from the C-terminal region of the human EphA2 Receptor protein.
PBS, pH 7.4
0.1% sodium azide
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Have you cited this antibody in a publication?
so we can reference it in this datasheet.
Protein Aliases: ARCC2; CTPA; CTPP1; ECK; ephrin receptor EphA2; Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2
Gene Aliases: ARCC2; AW545284; CTPA; CTPP1; CTRCT6; ECK; EPHA2; Myk2; Sek-2; Sek2
UniProt ID: (Human) P29317, (Mouse) Q03145
Entrez Gene ID: (Human) 1969, (Mouse) 13836, (Rat) 366492
Suggested Secondary Antibodies
Material safety data sheets (MSDS)