Invitrogen

EphA4 Monoclonal Antibody (4C8H5)

Advanced Verification

17 Published Figures

37 References

View all (30) EphA4 antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Additional Information:

Published Figures (17)
Advanced Verification (1)

EphA4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

EphA4 Antibody (37-1600) in ICC/IF

Molecular and cellular biology 2013 - Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Immunocytochemistry

EphA4 Antibody in Immunohistochemistry (IHC)

EphA4 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

EphA4 Antibody — Fig. 1 Reduction of EphA4 protein and mRNA levels in the hippocampus and cortex of APPPS1 mice. Representative images ( a ) and quantification ( b ) of a Western blot analysis of hippocampal EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot) with GAPDH protein levels as a loading control (unpaired t test and Mann-Whitney test respectively, n = 4-8 mice/group). Representative images ( c ) and quantification ( d ) of a Western blot analysis of cortical EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot). GAPDH protein levels were assessed to control for equal loading (unpaired t test, n = 4 mice/group). e Representative images of RNA scope with specific probes for EphA4 in the DG, CA3, and CA1 regions of the hippocampus of Ctrl and EphA4-KO mice. Hoechst was used to stain cell nuclei. * p <= 0.05, ** p <= 0.01. Scale bar = 100 mum. Abbreviation: Ctrl control {KO}

Immunogen image

Immunogen Image EphA4 Monoclonal Antibody (4C8H5)

Product Details

37-1600

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 28 publications 28 publications

Immunohistochemistry (IHC)

3-10 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

5 µg

View 3 publications 3 publications

Inhibition Assays (Inhibition)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4C8H5

Immunogen

A synthetic peptide derived from the C-terminus human EphA4 receptor protein.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533301

Product Specific Information

This antibody is specific for the endogenous EphA4 receptor protein. No cross-reactivity with EphA5 or EphA7 has been detected. On western blots, this antibody identifies a band at ~110-120 kDa. Reactivity has been confirmed with EphA4-transfected 293T cells and mouse brain lysates by western blot and immunoprecipitation and on transfected 293 cells and mouse hippocampus by immunohistochemistry.

Target Information

EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cek8; EK8; epa4; EPH-like kinase 8; Eph-related receptor protein tyrosine kinase; receptor protein-tyrosine kinase HEK8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase receptor MPK-3; tyrosine-protein kinase receptor SEK; tyrosine-protein kinase receptor SEK-1; tyrosine-protein kinase TYRO1; unnamed protein product

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Gene Aliases: 2900005C20Rik; Cek8; EK8; HEK8; rb; SEK; Sek1; TYRO1

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UniProt ID: (Chicken) Q07496, (Human) P54764, (Mouse) Q03137

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Entrez Gene ID: (Chicken) 395559, (Human) 2043, (Mouse) 13838

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Function(s)

protein kinase activity transmembrane receptor protein tyrosine kinase activity GPI-linked ephrin receptor activity transmembrane-ephrin receptor activity ATP binding PH domain binding identical protein binding DH domain binding protein tyrosine kinase binding beta-amyloid binding protein tyrosine kinase activity ephrin receptor activity protein binding kinase activity ephrin receptor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EphA4 Monoclonal Antibody (4C8H5)

EphA4 Antibody (37-1600) in ICC/IF

Product Details

37-1600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Inhibition Assays (Inhibition)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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