Immunohistochemistry analysis of Ephb3 in 4% PFA, OCT embedded frozen mouse E16.5 lung sections. Sections were blocked and probed with an Ephb3 mouse monoclonal antibody (Product # MA5-15394) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (A11004).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant fragment of EphB3 (aa39-212) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15394 targets EphB3 in IHC and WB applications and shows reactivity with Human samples.
The MA5-15394 immunogen is purified recombinant fragment of EphB3 (aa39-212) expressed in E. Coli.
MA5-15394 has been successfully used in immunohistochemistry analysis of Ephb3 in mouse E16.5 frozen lung sections.
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase family. This gene encodes a receptor for ephrin-B family members.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.