|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||3-5 µg/mL|
|Immunofluorescence (IF)||3-5 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 2 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein corresponding to the C-terminal sequence of the human EphB4 receptor|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
37-1800 has been successfully used in Western Blot, immunohistochemical, immunofluorescence, and ELISA assays.
35-2900 by western blot detects a ~125kDa band representing EphB4 but also detects a strong band at ~90kDa , likely representing another protein in the ephrin family. 37-1800 is recommended for WB.
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; HTK; MYK1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; TYRO11; tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11
Gene Aliases: EPHB4; HTK; MYK1; TYRO11
UniProt ID: (Human) P54760
Entrez Gene ID: (Human) 2050
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