Invitrogen

EphB4 Monoclonal Antibody (3D7G8)

3 Published Figures

10 References

View all (16) EphB4 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

EphB4 Antibody — Figure 2 N/T scorings diagram for the tissue microarray (TMA) stained for EphA2 and EphB4. Green = higher score in tumor than normal tissue; Red = higher score in normal tissue than tumor tissue; Blue = no difference in score between normal and tumor tissue. The thickness of the line represents the number of patients, the thicker the line the more patients. The numbers 0-7 represent the IHC scoring values.

EphB4 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of EphB4 (green) in HT29 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a EphB4 mouse monoclonal antibody (Product # 37-1800) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

EphB4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of human colon carcinoma tissue using EphB4 Receptor Monoclonal Antibody, Mouse (clone 3D7G8) (clone 3D7G8) (Product # 37-1800).

EphB4 Antibody in Western Blot (WB) — Western blot analysis of EphB4 was performed by loading 20 µg of indicated whole cell lysate and 7 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. EphB4 was detected at ~125 kDa using a EphB4 mouse monoclonal antibody (Product # 37-1800, upper panel) at a concentration of 2 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:2000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076). GAPDH was detected at ~37 kDa using a GAPDH rabbit polyclonal antibody, HRP conjugate (Product # PA1-987-HRP, lower panel).

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay Dependent

Immunocytochemistry (ICC)

3-5 µg/mL

Immunofluorescence (IF)

3-5 µg/mL

Immunohistochemistry (IHC)

Assay Dependent

Immunoprecipitation (IP)

Assay Dependent

Western Blot (WB)

1-2 µg/mL

Published Applications

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Peptide Array (Array)

See 1 publication below

Flow Cytometry (Flow)

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Miscellaneous PubMed (Misc)

See 2 publications below

Western Blot (WB)

See 2 publications below

Immunoprecipitation (IP)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D7G8

Immunogen

Recombinant protein derived from the C-terminal region of the human EphB4 protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533303

Product Specific Information

37-1800 has been successfully used in Western Blot, immunohistochemical, immunofluorescence, ELISA and immunoprecipitation assays. By western blot this antibody detects a ~125kDa protein representing EPHB4 in MCF7, HT-29 and U2OS lysate.

Target Information

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; HTK; MYK1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; TYRO11; tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

Gene Aliases: EPHB4; HTK; MYK1; TYRO11

UniProt ID: (Human) P54760

Entrez Gene ID: (Human) 2050

Function(s)

angiogenesis cell migration involved in sprouting angiogenesis heart morphogenesis cell adhesion peptidyl-tyrosine phosphorylation protein autophosphorylation ephrin receptor signaling pathway

Process(es)

transmembrane receptor protein tyrosine kinase activity ephrin receptor activity protein binding ATP binding