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      EphB4 Monoclonal Antibody (3D7G8)

      See 11 other EphB4 antibodies ›
      EphB4 Monoclonal Antibody for Western Blot, IF, ICC, IHC, IP and ELISA

      Catalog#: 37-1800 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      37-1800 100 µg
      304.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Mouse Secondary Antibodies (597)
      Alexa Fluor® Anti-Mouse Secondary Antibodies (122)
      HRP Anti-Mouse Secondary Antibodies (86)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human
      Published species reactivity Hamster, Human, Mouse, Not Applicable
      Host / Isotype Mouse / IgG1, kappa
      Class Monoclonal
      Type Antibody
      Clone 3D7G8
      Immunogen Recombinant protein derived from the C-terminal region of the human EphB4 protein.
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.5 mg/ml
      Purification Protein A
      Storage buffer PBS, pH 7.4
      Contains 0.1% sodium azide
      Storage Conditions -20°C
      Tested Applications Dilution *
      ELISA (ELISA) Assay Dependent
      Immunocytochemistry (ICC) 3-5 µg/mL
      Immunofluorescence (IF) 3-5 µg/mL
      Immunohistochemistry (IHC) Assay Dependent
      Immunoprecipitation (IP) Assay Dependent
      Western Blot (WB) 1-2 µg/ml

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Miscellaneous PubMed (MISC) See 2 publications below
      Western Blot (WB) See 2 publications below
      Immunoprecipitation (IP) See 1 publications below

      Product Specific Information

      37-1800 has been successfully used in Western Blot, immunohistochemical, immunofluorescence, ELISA and immunoprecipitation assays. By western blot this antibody detects a ~125kDa protein representing EPHB4 in MCF7, HT-29 and U2OS lysate.

      Background/Target Information

      Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Miscellaneous PubMed

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited YES oncogenic activity is specified by its SH4 domain and regulates RAS/MAPK signaling in colon carcinoma cells.
      37-1800 was used in immunoprecipitation to elucidate how YES contributes to colorectal cancer progression       		Dubois F,Leroy C,Simon V,Benistant C,Roche S American journal of cancer research (5:1972) 2015
      Human 10 µg/ml Gene expression profiling identifies EPHB4 as a potential predictive biomarker in colorectal cancer patients treated with bevacizumab.
      37-1800 was used in immunohistochemistry (paraffin) to investigate the prognostic value of EPHB4 in colorectal cancer therapy.       		Guijarro-Muñoz I,Sánchez A,Martínez-Martínez E,García JM,Salas C,Provencio M,Alvarez-Vallina L,Sanz L Medical oncology (Northwood, London, England) (30:null) 2013

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable 1:200 EPHB4 and survival of colorectal cancer patients.
      37-1800 was used in immunohistochemistry - paraffin section and western blot to study the role of EPHB4 in colorectal cancer and determine its value as a prognostic marker       		Davalos V,Dopeso H,Castaño J,Wilson AJ,Vilardell F,Romero-Gimenez J,Espín E,Armengol M,Capella G,Mariadason JM,Aaltonen LA,Schwartz S,Arango D Cancer research (66:8943) 2006
      Hamster
      Mouse       		Not Cited
      Not Cited       		EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity. Koolpe M,Burgess R,Dail M,Pasquale EB The Journal of biological chemistry (280:17301) 2005

      Immunoprecipitation

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Hamster
      Mouse
      		Not Cited
      Not Cited
      		EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity. Koolpe M,Burgess R,Dail M,Pasquale EB The Journal of biological chemistry (280:17301) 2005

      Bioinformatics

      Protein Aliases:

      ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; HTK; MYK1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; TYRO11; tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

      Gene Aliases:

      EPHB4; HTK; MYK1; TYRO11

      UniProt ID:

      (Human) P54760

      Entrez Gene ID:

      (Human) 2050

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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