Immunofluorescence analysis of Ephrin-A3 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin-A3 Rabbit Polyclonal Antibody (Product # 36-7500) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminus of mouse ephrin-A3 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Ephrin receptor EphA4 restricts axonal sprouting and enhances branching in the injured mouse optic nerve.
36-7500 was used in immunohistochemistry - frozen section and western blot to investigate the role of EphA4 and EphrinA3 in adult axon regeneration using a model of adult mouse optic nerve injury
|Joly S,Jordi N,Schwab ME,Pernet V||The European journal of neuroscience (40:3021)||2014|
Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration.
36-7500 was used in immunohistochemistry - free floating and western blot to study the increased levels of certain axon guidance proteins in the CNS of Nogo-A ablated mice and their role in limiting the outgrowth of neurites in the absence of Nogo.
|Kempf A,Montani L,Petrinovic MM,Schroeter A,Weinmann O,Patrignani A,Schwab ME||The European journal of neuroscience (38:3567)||2013|
|Mouse||Not Cited||Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.||Murai KK,Nguyen LN,Irie F,Yamaguchi Y,Pasquale EB||Nature neuroscience (6:153)||2003|