|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human ephrin A4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.
Ephrin A4 (the antigen to this antibody) is also known as LERK-4 or ELF-4. Ephrin-A4 binds EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. To date, at least 14 members of the Eph receptor family and a family of 8 ligands have been identified.
Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses: ephrin-A and ephrin-B. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositollinkages; whereas ephrin-B subclass consists of ligands with transmembrane domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.