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Invitrogen
Ephrin B (Pan) Monoclonal Antibody (2D3E9)
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FIGURE: 1 / 7
Ephrin B (Pan) Antibody (37-8100) in ICC/IF
Immunofluorescent analysis of Ephrin-B Pan was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin-B Pan (2D3E9) Mouse Monoclonal Antibody (Product # 37-8100) at 2 µg/mL in 0.1% BSA and incubated for ... View More
Product Details
37-8100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2D3E9
Immunogen
Synthetic peptide derived from the ephrin-B sequence.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Ephrin B genes, including Ephrin B1, B2, and B3, encode membrane-bound ligands that interact with Eph receptors, particularly EphB1 through EphB6. These ligands are crucial for various developmental processes, notably in the cardiovascular and nervous systems. Ephrin B2 is prominently expressed in arterial endothelial cells, while its receptor EphB4 is found in venous endothelial cells, highlighting a key distinction early in vascular development. Disruption of these genes can lead to significant vascular remodeling failures during embryogenesis. Ephrin-Eph receptor signaling involves the binding of ephrin molecules to their receptors on adjacent cells, initiating heterodimerization and receptor autophosphorylation, followed by recruitment of signaling clusters. This signaling is essential for various physiological and pathological functions, including heart development and function, as well as the formation and maintenance of the glomerular tuft in kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CEK5 ligand; CEK5-L; EFL-3; ELK ligand; ELK-L; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 8; EphB receptor ligand; Ephrin-B1; Ephrin-B2; Ephrin-B3; HTK ligand; HTK-L; LERK-2; LERK-5; LERK-8; ligand of eph-related kinase 5; transmembrane ligand
Gene Aliases: CFND; CFNS; EFB1; EFL3; EFL6; EFNB1; EFNB2; EFNB3; Elk-L; EPLG2; EPLG5; EPLG8; Htk-L; HTKL; LERK2; LERK5; LERK8
UniProt ID: (Chicken) O73612, (Human) P98172, (Human) P52799, (Human) Q15768
Entrez Gene ID: (Chicken) 395896, (Human) 1947, (Chicken) 395625, (Human) 1948, (Human) 1949
nervous system development
cell differentiation
ephrin receptor signaling pathway
neural crest cell migration
cell adhesion
cell-cell signaling
axon guidance
embryonic pattern specification
T cell costimulation
positive regulation of T cell proliferation
lymph vessel development
cell migration involved in sprouting angiogenesis
anatomical structure morphogenesis
organ morphogenesis
negative regulation of keratinocyte proliferation
viral entry into host cell
regulation of chemotaxis
nephric duct morphogenesis
positive regulation of cardiac muscle cell differentiation
adult walking behavior
axon choice point recognition
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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