ErbB-2 (also known as Her-2 or neu) is a 185 kDa transmembrane receptor tyrosine kinase from the EGFR family that acts to regulate a variety of biological responses including cell growth, differentiation, and tissue development. ErbB-2 forms complexes with other members of the EGFR receptor family. Binding of growth factors to these related receptors leads to phosphorylation on several tyrosine residues within the cytoplasmic domain of ErbB-2, thus activating the receptor. A direct link between overexpression of ErbB-2 in breast and ovarian cancers, which account for one-third of all cancers in women and approximately one-quarter of cancer-related deaths in females, has been shown in the literature. In addition, overexpression or abnormal activation of ErbB-2 has been found in a variety of tumors including brain, lung, prostate and skin cancer.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human p185/HER-2.|
|Storage buffer||proprietary buffer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
ErbB2 (also known as Her2 or neu) is a 185 kDa transmembrane receptor tyrosine kinase from the EGFR family that acts to regulate a variety of biological responses including cell growth, differentiation, and tissue development. Ligand binding to the extracellular domain, or overexpression of the receptor leads to phosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Overexpression or abnormal activation of ErbB2 has been found in a variety of tumors including brain, breast, lung and skin cancer. Autophosphorylation of tyrosine 1139 on ErbB2 allows binding of Grb2 and the Src SH2 domain, which allows activation of the Ras, Raf, ERK1 and 2 and JAK/STAT signaling pathways, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of c-erbB-2 in breast cancer cell lines as experimental receptor of magnetic nanoparticles.
AHO0918 was used in flow cytometry to evaluate the expression of the c-erbB-2 gene and c-erbB-2 antigen in breast cancer cells type BT-474, MCF-7 and MDA-MB-23 as targets for magnetic nanoparticles.
|Silva JG,Sánchez V,Polo SM,González CA||Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference (2013:4498)||2013|