Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (N12), Biotin

Product Image Gallery

ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HER-2 (green) showing staining in the membrane of SK-BR-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a HER-2 monoclonal antibody (Product # MA5-12998) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ErbB2 (HER-2) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HER-2 showing positive staining in the cytoplasm, nucleus and membrane of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a HER-2 monoclonal antibody (Product # MA5-12998) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Product Details

MA5-12995

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:10-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 3 publications 3 publications

Immunomicroscopy (IM)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N12

Immunogen

Intact SKBR-3 breast cancer cells

View immunogen

Conjugate

Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10983436

Product Specific Information

MA5-12995 targets HER-2 in ICC/IF, IHC (F), IHC (P) and IM applications and shows reactivity with Human samples.

The MA5-12995 immunogen is intact SKBR-3 breast cancer cells.

Target Information

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Truncated ErbB2 expressed in tumor cell nuclei contributes to acquired therapeutic resistance to ErbB2 kinase inhibitors.

Molecular cancer therapeutics

Xia W,Liu Z,Zong R,Liu L,Zhao S,Bacus SS,Mao Y,He J,Wulfkuhle JD,Petricoin EF,Osada T,Yang XY,Hartman ZC,Clay TM,Blackwell KL,Lyerly HK,Spector NL

MA5-12995 was used in immunocytochemistry, immunoprecipitation, and western blot to study the role of truncated nuclear ErbB2 in acquired resistance to ErbB2 kinase inhibitors in tumor cells

Mon Aug 01 00:00:00 EDT 2011

Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action.

Breast cancer research : BCR

DeFazio-Eli L,Strommen K,Dao-Pick T,Parry G,Goodman L,Winslow J

MA5-12995 was used in immunoprecipitation to investigate the state of HER1-HER2 interaction and modification in cell lines and tumors

Fri Apr 15 00:00:00 EDT 2011

Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens.

Pathology research international

Larson JS,Goodman LJ,Tan Y,Defazio-Eli L,Paquet AC,Cook JW,Rivera A,Frankson K,Bose J,Chen L,Cheung J,Shi Y,Irwin S,Kiss LD,Huang W,Utter S,Sherwood T,Bates M,Weidler J,Parry G,Winslow J,Petropoulos CJ,Whitcomb JM

MA5-12995 was used in flow cytometry to validate a method for the measurement of HER2 total protein and HER2 homodimers in FFPE breast cancer tumor specimens

Mon Jun 28 00:00:00 EDT 2010

Silica-gold nanoshells as potential intraoperative molecular probes for HER2-overexpression in ex vivo breast tissue using near-infrared reflectance confocal microscopy.

Breast cancer research and treatment

Bickford LR,Agollah G,Drezek R,Yu TK

MA5-12995 was used in immunocytochemistry to study silica-gold nanoshells as potential probes for HER2-overexpression in ex vivo breast tissue

Thu Apr 01 00:00:00 EDT 2010

Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy.

Nanotechnology

Bickford L,Sun J,Fu K,Lewinski N,Nammalvar V,Chang J,Drezek R

MA5-12995 was used in immunocytochemistry to develop a method for multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells

Wed Aug 06 00:00:00 EDT 2008

Impact of common epidermal growth factor receptor and HER2 variants on receptor activity and inhibition by lapatinib.

Cancer research

Gilmer TM,Cable L,Alligood K,Rusnak D,Spehar G,Gallagher KT,Woldu E,Carter HL,Truesdale AT,Shewchuk L,Wood ER

MA5-12995 was used in immunoprecipitation to investigate the effect of lapatinib on cell proliferation regulated by EGFR/HER2

Tue Jan 15 00:00:00 EST 2008

Immunonanoshells for targeted photothermal ablation of tumor cells.

International journal of nanomedicine

Lowery AR,Gobin AM,Day ES,Halas NJ,West JL

MA5-12995 was used in immunocytochemistry to study the use of immunonanoshells for targeted photothermal ablation of tumor cells

Tue Sep 18 00:00:00 EDT 2007

Delineation of molecular mechanisms of sensitivity to lapatinib in breast cancer cell lines using global gene expression profiles.

Molecular cancer therapeutics

Hegde PS,Rusnak D,Bertiaux M,Alligood K,Strum J,Gagnon R,Gilmer TM

MA5-12995 was used in immunocytochemistry to examine the mechanism for the lapatinib sensitivity in breast cancer cell lines

Tue May 01 00:00:00 EDT 2007

Tumor cell expression of HLA-DM associates with a Th1 profile and predicts improved survival in breast carcinoma patients.

International immunology

Oldford SA,Robb JD,Codner D,Gadag V,Watson PH,Drover S

MA5-12995 was used in immunohistochemistry to study the predictive value of tumor cell expression of HLA-DM in breast carcinoma patients

Wed Nov 01 00:00:00 EST 2006

Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.

Cancer biotherapy & radiopharmaceuticals

Horak E,Heitner T,Robinson MK,Simmons HH,Garrison J,Russeva M,Furmanova P,Lou J,Zhou Y,Yuan QA,Weiner LM,Adams GP,Marks JD

MA5-12995 was used in western blot to develop and characterize specific antibodies targeting EGFR family

Thu Dec 01 00:00:00 EST 2005

Truncated ErbB2 receptor (p95ErbB2) is regulated by heregulin through heterodimer formation with ErbB3 yet remains sensitive to the dual EGFR/ErbB2 kinase inhibitor GW572016.

Oncogene

Xia W,Liu LH,Ho P,Spector NL

MA5-12995 was used in immunoprecipitation and western blot to study the mechanisms underlying the regulation of truncated ErbB2 receptor by heregulin

Thu Jan 22 00:00:00 EST 2004

C-erbB2 oncoprotein and its soluble ectodomain: a new potential tumor marker for prognosis early detection and monitoring patients undergoing Herceptin treatment.

Clinica chimica acta; international journal of clinical chemistry

Wu JT

MA5-12995 was used in ELISA to investigate the prognostic value of C-erbB2 oncoprotein in Herceptin treatment

Thu Aug 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: c-erb B2/neu protein; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

Gene Aliases: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1

UniProt ID: (Human) P04626

Entrez Gene ID: (Human) 2064

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

RNA polymerase I core binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding protein C-terminus binding growth factor binding protein phosphatase binding identical protein binding ErbB-3 class receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity protein dimerization activity

ErbB2 (HER-2) Monoclonal Antibody (N12), Biotin

Product Details

MA5-12995

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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