Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (e2-4001)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

12 References

View all (273) Her-2 (ErbB2) antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

ErbB2 (HER-2) Antibody in Western Blot (WB)

FIGURE: 1 / 8

ErbB2 (HER-2) Antibody (AHO1011) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (1), T-47D (2) and MDA-MB-231 (3). The blot was probed with anti-ErbB2 Mouse Monoclonal Antibody (Product # AHO1011, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 and moderate in T-47D where as it was not observed in MDA-MB-231 which is an ErbB2 negative cell... View More

ErbB2 (HER-2) Antibody in Immunohistochemistry (IHC)

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Product Details

AHO1011

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

e2-4001

Immunogen

Cytoplasmic domain of human c-erbB-2 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Storage conditions

-20°C

Shipping conditions

Dry ice

RRID

AB_2536320

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HDAC5-mediated deacetylation and nuclear localisation of SOX9 is critical for tamoxifen resistance in breast cancer.

British journal of cancer

Xue Y,Lian W,Zhi J,Yang W,Li Q,Guo X,Gao J,Qu H,Lin W,Li Z,Lai L,Wang Q

AHO1011 was used in Western Blotting to study the mechanisms underlying the regulation of SOX9 during tamoxifen resistance.

Sun Dec 01 00:00:00 EST 2019

SOX9/FXYD3/Src Axis Is Critical for ER⁺ Breast Cancer Stem Cell Function.

Molecular cancer research : MCR

Xue Y,Lai L,Lian W,Tu X,Zhou J,Dong P,Su D,Wang X,Cao X,Chen Y,Wang Q

AHO1011 was used in Western Blotting to show that an estrogen-inducible gene FXYD3, whose expression is significantly upregulated in ER+ breast CSCs, is a critical player for regulating ER+ breast CSC function.

Tue Jan 01 00:00:00 EST 2019

Molecular Correlates of In Vitro Responses to Dacomitinib and Afatinib in Bladder Cancer.

Bladder cancer (Amsterdam, Netherlands)

Tamura S,Wang Y,Veeneman B,Hovelson D,Bankhead A,Broses LJ,Lorenzatti Hiles G,Liebert M,Rubin JR,Day KC,Hussain M,Neamati N,Tomlins S,Palmbos PL,Grivas P,Day ML

AHO1011 was used in western blotting to detect levels of erbB2 in bladder cancer cell lines.

Sat Jan 20 00:00:00 EST 2018

Integrated Molecular Characterization of Uterine Carcinosarcoma.

Cancer cell

Cherniack AD,Shen H,Walter V,Stewart C,Murray BA,Bowlby R,Hu X,Ling S,Soslow RA,Broaddus RR,Zuna RE,Robertson G,Laird PW,Kucherlapati R,Mills GB,Weinstein JN,Zhang J,Akbani R,Levine DA

AHO1011 was used in reverse phase protein array to characterize the molecular identity of uterine carcinosarcomas.

Mon Mar 13 00:00:00 EDT 2017

Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling.

Cell systems

Hill SM,Nesser NK,Johnson-Camacho K,Jeffress M,Johnson A,Boniface C,Spencer SE,Lu Y,Heiser LM,Lawrence Y,Pande NT,Korkola JE,Gray JW,Mills GB,Mukherjee S,Spellman PT

AHO1011 was used in western blot to analyze the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches

Wed Jan 25 00:00:00 EST 2017

Use of a genetically engineered mouse model as a preclinical tool for HER2 breast cancer.

Disease models & mechanisms

Creedon H,Balderstone LA,Muir M,Balla J,Gomez-Cuadrado L,Tracey N,Loane J,Klinowska T,Muller WJ,Brunton VG

AHO1011 was used in immunohistochemistry - paraffin section to determine the mechanisms of resistance to the pan-HER family inhibitor AZD8931

Mon Feb 01 00:00:00 EST 2016

Use of a genetically engineered mouse model as a preclinical tool for HER2 breast cancer.

Disease models & mechanisms

Creedon H,Balderstone LA,Muir M,Balla J,Gomez-Cuadrado L,Tracey N,Loane J,Klinowska T,Muller WJ,Brunton VG

AHO1011 was used in immunohistochemistry - paraffin section to determine the mechanisms of resistance to the pan-HER family inhibitor AZD8931

Mon Feb 01 00:00:00 EST 2016

A Novel Affibody-Auristatin E Conjugate With a Potent and Selective Activity Against HER2+ Cell Lines.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Sochaj-Gregorczyk AM,Serwotka-Suszczak AM,Otlewski J

AHO1011 was used in western blot to develop a new affibody-drug conjugate against HER2-positive cell lines.

Thu Jan 04 00:00:00 EST 2018

Molecular architecture of the ErbB2 extracellular domain homodimer.

Oncotarget

Hu S,Sun Y,Meng Y,Wang X,Yang W,Fu W,Guo H,Qian W,Hou S,Li B,Rao Z,Lou Z,Guo Y

AHO1011 was used in immunoprecipitation and western blot to elucidate the dimerization of ErbB2

Fri Jan 30 00:00:00 EST 2015

Pertuzumab counteracts the inhibitory effect of ErbB2 on degradation of ErbB3.

Carcinogenesis

Sak MM,Szymanska M,Bertelsen V,Hasmann M,Madshus IH,Stang E

AHO1011 was used in immunocytochemistry and western blot to investigate factors that regulate ErbB2-ErbB3 heterodimerization.

Sun Sep 01 00:00:00 EDT 2013

Pertuzumab counteracts the inhibitory effect of ErbB2 on degradation of ErbB3.

Carcinogenesis

Sak MM,Szymanska M,Bertelsen V,Hasmann M,Madshus IH,Stang E

AHO1011 was used in immunocytochemistry and western blot to investigate factors that regulate ErbB2-ErbB3 heterodimerization.

Sun Sep 01 00:00:00 EDT 2013

Glycerophospholipid profile in oncogene-induced senescence.

Biochimica et biophysica acta

Cadenas C,Vosbeck S,Hein EM,Hellwig B,Langer A,Hayen H,Franckenstein D,Büttner B,Hammad S,Marchan R,Hermes M,Selinski S,Rahnenführer J,Peksel B,Török Z,Vígh L,Hengstler JG

AHO1011 was used in flow cytometry to study the influence of oncogene expression on cellular lipid profile.

Sat Sep 01 00:00:00 EDT 2012

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ErbB2 (HER-2) Monoclonal Antibody (e2-4001)

ErbB2 (HER-2) Antibody (AHO1011) in WB

Product Details

AHO1011

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Target Information

Performance Guarantee

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