Whole cell lysates of serum starved MDA-MB231 (1), MDA-MB468 (2), MCF-7 (3), T47D (4), SW480 (5) and SW620 (6) tumor cells (approximately 20,000 cells per lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-ErbB3/Her3 (C-term) antibody (Catalog no. 44897M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detected using an ECL detection method (exposure time: 30 seconds).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Peptide conjugated to hemocyanin. This antibody specifically recognizes the C-terminus of ErbB3/Her3 (185 kDa).|
|Storage buffer||PBS, pH 7.3, with PEG, sucrose|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. C-erbB-3 is over-expressed in a variety of tumors inculding breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.