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Immunofluorescence analysis of ErbB3 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ErbB3 (RTJ2) Mouse Monoclonal Antibody (MA1860) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic Peptide: E(1004) L E P E L D L D L D L E(1016)|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||0.5-2 ug per 10^6 cells|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (IHC)||6 - 8 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-860 detects Her-3 from human and mouse samples.
MA1-860 has been successfully used in FACS, immunoprecipitation, immunohistochemical, immunofluorescence and ELISA procedures. By immunoprecipitation, this antibody detects ~185 kDa protein, representing Her-3 from human tissue extracts.This antibody has also been used in immunohistochemical, immunofluorescence and ELISA procedures using human tissues.
MA1-860 immunizing peptide corresponds to amino acid residues 1004 to 1016 of human cytoplasmic domain of HER-3 protein.
Her-3, also known as c-erbB-3, is a Type 1 membrane protein found in numerous cell types. It is a member of the EGF receptor protein family, and is activated by neuregulins and NTAK. Her-3 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies. Her-3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neuregulin expression, function, and signaling in human ovarian cancer cells.
MA1-860 was used in western blot to investigate the characteristics of neuregulin in human ovarian cancer cells
|Gilmour LM,Macleod KG,McCaig A,Sewell JM,Gullick WJ,Smyth JF,Langdon SP||Clinical cancer research : an official journal of the American Association for Cancer Research (8:3933)||2002|
avian erythroblastosis oncogene B 3 receptor; c-erbB-3; C-errB-3; ERBB3; glial growth factor receptor; human epidermal growth factor receptor 3; MDA BF 1; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; receptor tyrosine protein kinase ERB3; receptor tyrosine-protein kinase erbB-3; tyrosine kinase type cell surface receptor HER3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
c-erbB-3; c-erbB3; C76256; ErbB-3; ERBB3; erbB3-S; Erbb3r; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3