Invitrogen

ErbB3 Monoclonal Antibody (2F12)

Advanced Verification

IP-mass spectrometry

2 Published Figures

27 References

View all (83) Her-3 (ErbB3) antibodies

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ErbB3 Antibody (MA5-12675) in WB

Knockout of ErbB3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR764358_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of ErbB3 was performed by loading 30 µg of T-47D Cas9 (Lane 1) andT-47D ErbB3 KO (Lane 2) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotti... View More

ErbB3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ErbB3 Antibody — IP-MS enrichment of ERBB3 (LFQ intensity): ERBB3 was enriched 525-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and ERBB3 antibody (Product # MA5-12675). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

MA5-12675

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

View 5 publications 5 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2F12

Immunogen

Recombinant rat c-erbB-3/HER-3 oncoprotein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11001597

Product Specific Information

MA5-12675 targets HER-3 in WB applications and shows reactivity with Bovine, Human, mouse, and Rat samples.

The MA5-12675 immunogen is recombinant rat c-erbB-3/HER-3 oncoprotein.

Target Information

ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HER2 inhibition increases non-muscle myosin IIA to promote tumorigenesis in HER2+ breast cancers.

PloS one

Alanazi SM,Feroz W,Mishra R,Kilroy MK,Patel H,Yuan L,Storr SJ,Garrett JT

MA5-12675 was used in Immunoprecipitation to identify proteins that bound to HER3 upon inhibition of the HER family with the pan-HER inhibitor neratinib in HER2+ breast cancer cells.

Tue May 23 00:00:00 EDT 2023

A bivalent, bispecific Dab-Fc antibody molecule for dual targeting of HER2 and HER3.

mAbs

Rau A,Kocher K,Rommel M,Kühl L,Albrecht M,Gotthard H,Aschmoneit N,Noll B,Olayioye MA,Kontermann RE,Seifert O

MA5-12675 was used in Western Blot to present a novel bivalent and bispecific antagonistic molecule (Dab-Fc) targeting human epidermal growth factors 2 and 3 (HER2 and HER3) derived from the Db-Ig platform, which was developed for the generation of multivalent and multispecific antibody molecules.

Wed Jan 19 00:00:00 EST 2022

Inhibition of Tumor Cell Growth and Cancer Stem Cell Expansion by a Bispecific Antibody Targeting EGFR and HER3.

Molecular cancer therapeutics

Rau A,Lieb WS,Seifert O,Honer J,Birnstock D,Richter F,Aschmoneit N,Olayioye MA,Kontermann RE

MA5-12675 was used in Western Blot to develop a novel bispecific antibody targeting EGFR and HER3 for the use as a potential cancer therapy.

Wed Jul 01 00:00:00 EDT 2020

Diabody-Ig: a novel platform for the generation of multivalent and multispecific antibody molecules.

mAbs

Seifert O,Rau A,Beha N,Richter F,Kontermann RE

MA5-12675 was used in Western Blotting to present a novel platform for the generation of Ig-like molecules, designated diabody-Ig (Db-Ig).

Mon Jul 01 00:00:00 EDT 2019

Ligand-associated ERBB2/3 activation confers acquired resistance to FGFR inhibition in FGFR3-dependent cancer cells.

Oncogene

Wang J,Mikse O,Liao RG,Li Y,Tan L,Janne PA,Gray NS,Wong KK,Hammerman PS

Published figure using ErbB3 monoclonal antibody (Product # MA5-12675) in Immunoprecipitation

Thu Apr 23 00:00:00 EDT 2015

Ligand-associated ERBB2/3 activation confers acquired resistance to FGFR inhibition in FGFR3-dependent cancer cells.

Oncogene

Wang J,Mikse O,Liao RG,Li Y,Tan L,Janne PA,Gray NS,Wong KK,Hammerman PS

Published figure using ErbB3 monoclonal antibody (Product # MA5-12675) in Immunoprecipitation

Thu Apr 23 00:00:00 EDT 2015

Additive impact of HER2-/PTK6-RNAi on interactions with HER3 or IGF-1R leads to reduced breast cancer progression in vivo.

Molecular oncology

Falkenberg N,Anastasov N,Höfig I,Bashkueva K,Lindner K,Höfler H,Rosemann M,Aubele M

MA5-12675 was used in immunohistochemistry to identify the effects of the epidermal growth factor receptor 2 and the protein tyrosine kinase 6 in relation to breast cancer treatment

Thu Jan 01 00:00:00 EST 2015

T47D breast cancer cells switch from ER/HER to HER/c-Src signaling upon acquiring resistance to the antiestrogen fulvestrant.

Cancer letters

Kirkegaard T,Hansen SK,Larsen SL,Reiter BE,Sørensen BS,Lykkesfeldt AE

MA5-12675 was used in immunoprecipitation to study the switch from ER/HER to HER/c-Src signaling as T47D breast cancer cells acquire fulvestrant resistance

Sat Mar 01 00:00:00 EST 2014

EGFR, HER2 and HER3 dimerization patterns guide targeted inhibition in two histotypes of esophageal cancer.

International journal of cancer

Fichter CD,Timme S,Braun JA,Gudernatsch V,Schöpflin A,Bogatyreva L,Geddert H,Faller G,Klimstra D,Tang L,Hauschke D,Werner M,Lassmann S

MA5-12675 was used in immunocytochemistry, immunohistochemistry, and western blot to study the value of the expression and dimerization patterns of EGFR, HER2 and HER3 in esophageal cancer histotypes for guiding targeted anti-EGFR and -HER2 therapy

Wed Oct 01 00:00:00 EDT 2014

HER2/HER3 heterodimers and p21 expression are capable of predicting adjuvant trastuzumab response in HER2+ breast cancer.

Breast cancer research and treatment

Green AR,Barros FF,Abdel-Fatah TM,Moseley P,Nolan CC,Durham AC,Rakha EA,Chan S,Ellis IO

MA5-12675 was used in proximity ligation assay to research p21 expression and HER2/HER3 heterodimers and prediction of adjuvant trastuzumab response in HER2+ breast cancer

Thu May 01 00:00:00 EDT 2014

Spontaneous and pronase-induced HER2 truncation increases the trastuzumab binding capacity of breast cancer tissues and cell lines.

The Journal of pathology

Recupero D,Daniele L,Marchiò C,Molinaro L,Castellano I,Cassoni P,Righi A,Montemurro F,Sismondi P,Biglia N,Viale G,Risio M,Sapino A

MA5-12675 was used in immunocytochemistry to study the effect of spontaneous and pronase-induced HER2 truncation on the trastuzumab binding capacity of breast cancer tissues and cell lines

Fri Feb 01 00:00:00 EST 2013

MiR-221/-222 differentiate prognostic groups in advanced breast cancers and influence cell invasion.

British journal of cancer

Falkenberg N,Anastasov N,Rappl K,Braselmann H,Auer G,Walch A,Huber M,Höfig I,Schmitt M,Höfler H,Atkinson MJ,Aubele M

MA5-12675 was used in western blot to discuss targeting MiR-221/-222 to treat breast cancer

Tue Nov 12 00:00:00 EST 2013

Epithelial-mesenchymal transition markers and HER3 expression are predictors of elisidepsin treatment response in breast and pancreatic cancer cell lines.

PloS one

Teixidó C,Marés R,Aracil M,Ramón y Cajal S,Hernández-Losa J

MA5-12675 was used in western blot to study the value of HER3 expression and markers of epithelial-mesenchymal transition in predicting sensitivity of breast and pancreatic cancer cell lines to elisidepsin

Thu Aug 15 00:00:00 EDT 2013

Strong EGFR signaling in cell line models of ERBB2-amplified breast cancer attenuates response towards ERBB2-targeting drugs.

Oncogenesis

Henjes F,Bender C,von der Heyde S,Braun L,Mannsperger HA,Schmidt C,Wiemann S,Hasmann M,Aulmann S,Beissbarth T,Korf U

MA5-12675 was used in western blot to investigate the role of EGFR signaling in the effects of ERBB2-targeting drugs

Mon Jul 02 00:00:00 EDT 2012

In situ detection of HER2:HER2 and HER2:HER3 protein-protein interactions demonstrates prognostic significance in early breast cancer.

Breast cancer research and treatment

Spears M,Taylor KJ,Munro AF,Cunningham CA,Mallon EA,Twelves CJ,Cameron DA,Thomas J,Bartlett JM

MA5-12675 was used in blocking or activating experiment to study prognostic value of HER2:HER2 and HER2:HER3 protein-protein interactions in breast cancer

Sun Apr 01 00:00:00 EDT 2012

An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells.

Cancer cell

Sheng Q,Liu X,Fleming E,Yuan K,Piao H,Chen J,Moustafa Z,Thomas RK,Greulich H,Schinzel A,Zaghlul S,Batt D,Ettenberg S,Meyerson M,Schoeberl B,Kung AL,Hahn WC,Drapkin R,Livingston DM,Liu JF

MA5-12675 was used in western blot to identify mechanisms of tumor growth inhibition in ovarian cancer

Tue Mar 16 00:00:00 EDT 2010

HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation.

Cancer research

Bollig-Fischer A,Dziubinski M,Boyer A,Haddad R,Giroux CN,Ethier SP

MA5-12675 was used in immunoprecipitation and western blot to study the role of HER-2 signaling in the regulation of biological networks associated with cell transformation

Fri Oct 15 00:00:00 EDT 2010

EGFR over-expression and activation in high HER2, ER negative breast cancer cell line induces trastuzumab resistance.

Breast cancer research and treatment

Dua R,Zhang J,Nhonthachit P,Penuel E,Petropoulos C,Parry G

MA5-12675 was used in western blot to study the effect of

Sun Aug 01 00:00:00 EDT 2010

HER kinase activation confers resistance to MET tyrosine kinase inhibition in MET oncogene-addicted gastric cancer cells.

Molecular cancer therapeutics

Bachleitner-Hofmann T,Sun MY,Chen CT,Tang L,Song L,Zeng Z,Shah M,Christensen JG,Rosen N,Solit DB,Weiser MR

MA5-12675 was used in immunohistochemistry to study the mechanism for the resistance of MET oncogene-addicted gastric cancer cells against MET inhibitors

Sat Nov 01 00:00:00 EDT 2008

All EGF(ErbB) receptors have preformed homo- and heterodimeric structures in living cells.

Journal of cell science

Tao RH,Maruyama IN

MA5-12675 was used in western blot to study the dimerization of epidermal growth factor receptors prior to epidermal growth factor binding in plasma membrane

Wed Oct 01 00:00:00 EDT 2008

Growth stimulation of non-small cell lung cancer cell lines by antibody against epidermal growth factor receptor promoting formation of ErbB2/ErbB3 heterodimers.

Molecular cancer research : MCR

Maegawa M,Takeuchi K,Funakoshi E,Kawasaki K,Nishio K,Shimizu N,Ito F

MA5-12675 was used in immunoprecipitation to investigate the effect of an anti-EGFR antibody on non-small cell lung cancer cell lines

Sun Apr 01 00:00:00 EDT 2007

ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells.

American journal of physiology. Lung cellular and molecular physiology

Zscheppang K,Liu W,Volpe MV,Nielsen HC,Dammann CE

MA5-12675 was used in immunocytochemistry and western blot to study the role of ErbB4 in regulating fetal surfactant phospholipid synthesis in primary fetal rat type II cells

Wed Aug 01 00:00:00 EDT 2007

ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells.

Pediatric pulmonology

Zscheppang K,Korenbaum E,Bueter W,Ramadurai SM,Nielsen HC,Dammann CE

MA5-12675 was used in immunocytochemistry to study erbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells

Fri Dec 01 00:00:00 EST 2006

Therapeutic targeting of multiple signaling pathways in malignant pleural mesothelioma.

Oncology

Mukohara T,Civiello G,Johnson BE,Janne PA

MA5-12675 was used in western blot to study the treatment of malignant pleural mesothelioma by targeting multiple signaling pathways

Tue Sep 20 00:00:00 EDT 2005

The neuregulin GGF2 attenuates free radical release from activated microglial cells.

Journal of neuroimmunology

Dimayuga FO,Ding Q,Keller JN,Marchionni MA,Seroogy KB,Bruce-Keller AJ

MA5-12675 was used in western blot to investigate the protective effect of neuregulin GGF2 against free radical release from activated microglial cells

Sat Mar 01 00:00:00 EST 2003

The tyrosine kinase inhibitor ZD1839 ("Iressa") inhibits HER2-driven signaling and suppresses the growth of HER2-overexpressing tumor cells.

Cancer research

Moasser MM,Basso A,Averbuch SD,Rosen N

MA5-12675 was used in western blot to study the inhibition of HER2-driven signaling and growth suppression of HER2-overexpressing tumor cells by Iressa

Mon Oct 01 00:00:00 EDT 2001

Heregulin-dependent activation of phosphoinositide 3-kinase and Akt via the ErbB2/ErbB3 co-receptor.

The Journal of biological chemistry

Hellyer NJ,Kim MS,Koland JG

MA5-12675 was used in Western Blotting to investigate phosphoinositide 3-kinase-dependent signalling pathways activated by the ErbB2/ErbB3 co-receptor in COS7 cells.

Fri Nov 09 00:00:00 EST 2001

Bioinformatics

Protein Aliases: avian erythroblastosis oncogene B 3; avian erythroblastosis oncogene B 3 receptor; c-erbB-3; c-erbB3; C-errB-3; Glial growth factor receptor; human epidermal growth factor receptor 3; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3

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Gene Aliases: c-erbB-3; c-erbB3; C76256; ErbB-3; ERBB3; erbB3-S; Erbb3r; HER3; LCCS2; MDA-BF-1; nuc-ErbB3; p180-ErbB3; p45-sErbB3; p85-sErbB3

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UniProt ID: (Human) P21860, (Mouse) Q61526, (Rat) Q62799

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Entrez Gene ID: (Bovine) 785655, (Human) 2065, (Mouse) 13867, (Rat) 29496

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Function(s)

protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding growth factor binding protein tyrosine kinase activator activity neuregulin binding identical protein binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups neuregulin receptor activity transmembrane signal receptor

Process(es)

MAPK cascade endocardial cushion development negative regulation of cell adhesion signal transduction transmembrane receptor protein tyrosine kinase signaling pathway peripheral nervous system development heart development negative regulation of signal transduction Schwann cell differentiation phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cranial nerve development ERBB2 signaling pathway wound healing regulation of cell proliferation negative regulation of neuron apoptotic process positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling negative regulation of secretion neuron apoptotic process positive regulation of protein tyrosine kinase activity positive regulation of calcineurin-NFAT signaling cascade extrinsic apoptotic signaling pathway in absence of ligand regulation of cell motility protein phosphorylation epidermal growth factor receptor signaling pathway phosphorylation positive regulation of glucose import axonogenesis skeletal muscle tissue development circadian rhythm response to wounding signal transduction by protein phosphorylation cellular response to insulin stimulus odontogenesis response to drug tongue development mammary gland involution

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ErbB3 Monoclonal Antibody (2F12)

ErbB3 Antibody (MA5-12675) in WB

Product Details

MA5-12675

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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