|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||0.5-2 ug per 10^6 cells|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (IHC)||6 - 8 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay Dependent|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic Peptide: E(1004) L E P E L D L D L D L E(1016)|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-860 detects Her-3 from human and mouse samples.
MA1-860 has been successfully used in FACS, immunoprecipitation, immunohistochemical, immunofluorescence and ELISA procedures. By immunoprecipitation, this antibody detects ~185 kDa protein, representing Her-3 from human tissue extracts.This antibody has also been used in immunohistochemical, immunofluorescence and ELISA procedures using human tissues.
MA1-860 immunizing peptide corresponds to amino acid residues 1004 to 1016 of human cytoplasmic domain of HER-3 protein.
Her-3, also known as c-erbB-3, is a Type 1 membrane protein found in numerous cell types. It is a member of the EGF receptor protein family, and is activated by neuregulins and NTAK. Her-3 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies. Her-3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian erythroblastosis oncogene B 3 receptor; c-erbB-3; C-errB-3; ERBB3; Glial growth factor receptor; human epidermal growth factor receptor 3; MDA BF 1; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; receptor tyrosine protein kinase ERB3; Receptor tyrosine-protein kinase erbB-3; tyrosine kinase type cell surface receptor HER3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
Gene Aliases: c-erbB-3; c-erbB3; C76256; ErbB-3; ERBB3; erbB3-S; Erbb3r; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3
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