Invitrogen

ErbB4 Monoclonal Antibody (H4.72.8 (Ab72))

3 Published Figures

21 References

View all (50) ErbB4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (3)

FIGURE: 1 / 7

ErbB4 Antibody (MA5-13016) in WB

Western blot analysis of HER-4 was performed by loading 25 µg of mouse brain cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a HER-4 monoclonal antibody (Product # MA5-13016) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results ... View More

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Product Details

MA5-13016

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 10 publications 10 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Ferret, Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

H4.72.8 (Ab72)

Immunogen

Extracellular domain of recombinant human c-erbB-4/HER-4 oncoprotein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10990351

Product Specific Information

MA5-13016 targets HER-4 in WB, FACS applications and shows reactivity with Human and Mouse samples.

The MA5-13016 immunogen is extracellular domain of recombinant human c-erbB-4/HER-4 oncoprotein.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ERBB and P-glycoprotein inhibitors break resistance in relapsed neuroblastoma models through P-glycoprotein.

Molecular oncology

Rösch L,Herter S,Najafi S,Ridinger J,Peterziel H,Cinatl J,Jones DTW,Michaelis M,Witt O,Oehme I

Published figure using ErbB4 monoclonal antibody (Product # MA5-13016) in Neutralization

Sun Jan 01 00:00:00 UTC 2023

Nrg1β Released in Remote Ischemic Preconditioning Improves Myocardial Perfusion and Decreases Ischemia/Reperfusion Injury via ErbB2-Mediated Rescue of Endothelial Nitric Oxide Synthase and Abrogation of Trx2 Autophagy.

Arteriosclerosis, thrombosis, and vascular biology

Kundumani-Sridharan V,Subramani J,Owens C,Das KC

MA5-13016 was used in Proximity Ligation Assay (PLA) to conclude that Nrg1β is a RIPC factor that interacts with endothelial ErbB2 and prevents its degradation, which in turn prevents Trx2 degradation due to phosphorylation and inactivation of ATG5 (autophagy-related 5) by ErbB2.

Sun Aug 01 00:00:00 UTC 2021

Arteriosclerosis, thrombosis, and vascular biology

Kundumani-Sridharan V,Subramani J,Owens C,Das KC

Sun Aug 01 00:00:00 UTC 2021

Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4).

Histopathology

Jay JI,Brunhoeber PS,Smith MH,Williams RR,Sugarman MC,Free HL,Tast DE

MA5-13016 was used in immunohistochemistry to study the utility of the 4B5 monoclonal antibody for clinical HER2 status determination in breast tissue expressing HER4

Fri Mar 01 00:00:00 UTC 2013

Time course and progression of wild type α-synuclein accumulation in a transgenic mouse model.

BMC neuroscience

Amschl D,Neddens J,Havas D,Flunkert S,Rabl R,Römer H,Rockenstein E,Masliah E,Windisch M,Hutter-Paier B

MA5-13016 was used in immunohistochemistry - frozen section to study the time course of behavioral deficits and neuropathology in PDGF-beta human wild type alpha-synuclein transgenic mice

Wed Jan 09 00:00:00 UTC 2013

Slow down to stay alive: HER4 protects against cellular stress and confers chemoresistance in neuroblastoma.

Cancer

Hua Y,Gorshkov K,Yang Y,Wang W,Zhang N,Hughes DP

MA5-13016 was used in blocking or activating experiment to study the mechanisms underlying the modulation of neuroblastoma cellular stress and chemoresistance by HER4

Mon Oct 15 00:00:00 UTC 2012

Neuregulin directly decreases voltage-gated sodium current in hippocampal ErbB4-expressing interneurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Janssen MJ,Leiva-Salcedo E,Buonanno A

MA5-13016 was used in immunohistochemistry to study the direct effects of neuregulin on voltage-gated sodium channel activity of hippocampal interneurones expressing ErbB4

Wed Oct 03 00:00:00 UTC 2012

Decorin, erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 regulation of semaphorin 3A in central nervous system scar tissue.

Brain : a journal of neurology

Minor KH,Bournat JC,Toscano N,Giger RJ,Davies SJ

MA5-13016 was used in blocking or activating experiment to study the role of decorin in suppressing semaphorin 3A expression within adult rat cerebral cortex scar tissue

Fri Apr 01 00:00:00 UTC 2011

Populations of radial glial cells respond differently to reelin and neuregulin1 in a ferret model of cortical dysplasia.

PloS one

Poluch S,Juliano SL

MA5-13016 was used in blocking or activating experiment to investigate the influence of reelin and NRG1 on radial glia cells in ferrets

Thu Oct 28 00:00:00 UTC 2010

Heparin-binding epidermal growth factor-like growth factor is a potent neurotrophic factor for PC12 cells.

Neuro-Signals

Zhou Y,Besner GE

MA5-13016 was used in blocking or activating experiment to investigate the effect of heparin-binding epidermal growth factor-like growth factor on neuronal functions

Thu Feb 09 00:00:00 UTC 2012

Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth.

Molecular and cellular biology

Proietti CJ,Rosemblit C,Beguelin W,Rivas MA,Díaz Flaqué MC,Charreau EH,Schillaci R,Elizalde PV

MA5-13016 was used in immunocytochemistry to investigate the role of Stat3 in the tumorigenic effect of heregulin/ErbB-2 in breast cells

Sun Mar 01 00:00:00 UTC 2009

Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor.

American journal of obstetrics and gynecology

Leach RE,Kilburn BA,Petkova A,Romero R,Armant DR

MA5-13016 was used in blocking or activating experiment to study the role of HB-EGF as a survival factor in human cytotrophoblast cells exposed to hypoxia/reoxygenation injury

Tue Apr 01 00:00:00 UTC 2008

Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.

Biology of reproduction

Wolff GS,Chiang PJ,Smith SM,Romero R,Armant DR

MA5-13016 was used in blocking or activating experiment to examine the anti-apoptotic effect of epidermal growth factor-like growth factors in human placental cytotrophoblast cells

Sun Jul 01 00:00:00 UTC 2007

Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.

Brain research bulletin

Longart M,Chatani-Hinze M,Gonzalez CM,Vullhorst D,Buonanno A

MA5-13016 was used in immunocytochemistry to study the role of neuregulin in modulating ErbB4 endocytosis in cultured hippocampal neurons

Thu Jul 12 00:00:00 UTC 2007

Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.

Development (Cambridge, England)

Armant DR,Kilburn BA,Petkova A,Edwin SS,Duniec-Dmuchowski ZM,Edwards HJ,Romero R,Leach RE

MA5-13016 was used in blocking or activating experiment to study the role of metalloproteinase-mediated shedding of HB-EGF-like growth factor in human trophoblast survival at low oxygen concentrations

Wed Feb 01 00:00:00 UTC 2006

Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.

Cancer biotherapy & radiopharmaceuticals

Horak E,Heitner T,Robinson MK,Simmons HH,Garrison J,Russeva M,Furmanova P,Lou J,Zhou Y,Yuan QA,Weiner LM,Adams GP,Marks JD

MA5-13016 was used in western blot to develop and characterize specific antibodies targeting EGFR family

Thu Dec 01 00:00:00 UTC 2005

An inhibitor of the EGF receptor family blocks myeloma cell growth factor activity of HB-EGF and potentiates dexamethasone or anti-IL-6 antibody-induced apoptosis.

Blood

Mahtouk K,Jourdan M,De Vos J,Hertogh C,Fiol G,Jourdan E,Rossi JF,Klein B

MA5-13016 was used in immunoprecipitation to study the effect of an epidermal growth factor receptor inhibibor on HB-EGF activity and apoptosis

Mon Mar 01 00:00:00 UTC 2004

Signaling pathways required for matrix metalloproteinase-9 induction by betacellulin in head-and-neck squamous carcinoma cells.

International journal of cancer

O-charoenrat P,Wongkajornsilp A,Rhys-Evans PH,Eccles SA

MA5-13016 was used in blocking or activating experiment to investigate the mechanism of MMP9 induction by betacellulin in head-and-neck squamous carcinoma cells

Fri Aug 20 00:00:00 UTC 2004

Neuregulin expression, function, and signaling in human ovarian cancer cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gilmour LM,Macleod KG,McCaig A,Sewell JM,Gullick WJ,Smyth JF,Langdon SP

MA5-13016 was used in immunocytochemistry to investigate the characteristics of neuregulin in human ovarian cancer cells

Sun Dec 01 00:00:00 UTC 2002

Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.

Cancer research

O-charoenrat P,Modjtahedi H,Rhys-Evans P,Court WJ,Box GM,Eccles SA

MA5-13016 was used in flow cytometry to investigate the regulation of matrix metalloproteinase 9 by epidermal growth factor-like ligands in head and neck squamous carcinoma cells

Tue Feb 15 00:00:00 UTC 2000

Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation.

The EMBO journal

Elenius K,Paul S,Allison G,Sun J,Klagsbrun M

Published figure using ErbB4 monoclonal antibody (Product # MA5-13016) in Western Blot

Mon Mar 17 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

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Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4

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UniProt ID: (Human) Q15303, (Mouse) Q61527

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Entrez Gene ID: (Human) 2066, (Mouse) 13869

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Function(s)

protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding protein binding ATP binding protein homodimerization activity transcription regulatory region DNA binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

Performance Guarantee

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ErbB4 Monoclonal Antibody (H4.72.8 (Ab72))

ErbB4 Antibody (MA5-13016) in WB

Product Details

MA5-13016

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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