Immunofluorescent analysis of Estrogen Receptor (ER, green) in T47D and Hs 578T breast cancer cells. The cells were fixed with 4% paraformaldehyde for 15 min at room temp, permeabilized with 0.1% Triton X-100 for 15 min at room temp, and blocked with 0.3% BSA in PBS for at least 30 min at room temp. Cells were stained with an ER polyclonal antibody (Product # PA5-16476) at a dilution of 1:100 in blocking buffer overnight at 4C, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:500 for at least 1 hour at room temp. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Bovine, Human, Mouse, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the N-terminus of human estrogen receptor, alpha protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10-20 µg/mL|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||2.5-5.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16476 targets Estrogen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with mouse, Rabbit, Rat, Bovine, Human, Ovine, and Porcine samples.
The PA5-16476 immunogen is a synthetic peptide from the N-terminus of human estrogen receptor, alpha protein.
Estrogen Receptors (ER) are members of the steroid family of nuclear receptors. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Each protein contains distinct functional domains required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to EREs. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are several different isoforms of both ER alpha and ER beta. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Anti-inflammatory effects of a Chinese herbal medicine in atherosclerosis via estrogen receptor ß mediating nitric oxide production and NF-¿B suppression in endothelial cells.
PA5-16476 was used in western blot to study the roles of ER-beta, eNOS and NFkB in the mechanism by which Bu-Shen-Ning-Xin Decoction mediates its anti-inflammatory effects in atherosclerosis
|Wang L,Qiu XM,Hao Q,Li DJ||Cell death and disease (4:null)||2013|
The scaffold protein MEK Partner 1 is required for the survival of estrogen receptor positive breast cancer cells.
PA5-16476 was used in western blot to study the role of MEK Partner 1 in the survival of ER-positive breast cancer cells using siRNA-mediated knockdown
|Marina M,Wang L,Conrad SE||Cell communication and signaling : CCS (10:null)||2012|
Testosterone administration to adult rats differentially modulates androgen and oestrogen receptor-¿ expression in reproductive organs and pituitary.
PA5-16476 was used in immunohistochemistry and western blot to study the effect of testosterone administration on androgen and oestrogen receptor-alpha expression in reproductive organs and pituitary of rats
|Kaushik MC,Misro MM,Sehgal N,Nandan D||Andrologia (44 Suppl 1:312)||2012|