|ChIP assay (ChIP)||Assay Dependent|
|Gel Shift (GS)||1mg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:200-1:1000|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|ChIP assay (ChIP)||See 20 publications below|
|Immunoprecipitation (IP)||See 7 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Gel Shift (GS)||See 4 publications below|
|Western Blot (WB)||See 11 publications below|
|Immunocytochemistry (ICC)||See 6 publications below|
|Neutralization (Neu)||See 1 publications below|
|Tested Species reactivity||Bovine, Dog, Chicken, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Pig , Rat , Bovine , Human , Mouse , Chicken|
|Host / Isotype||Mouse / IgG1|
|Immunogen||35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13065 targets Estrogen Receptor in ChIP, GS, IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Hamster, Human, mouse, Ovine, Porcine, Rabbit, and Rat samples. The TE111.5D11 clone was developed against the entire ER ligand binding domain (LBD), but has greatest affinity for ER-alpha. Because ER-alpha and ER-beta share 50–60% homology, MA5-13065 recognizes ER-beta1 with lower affinity.
The MA5-13065 immunogen is 35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli.
Estrogen Receptors (ER) are members of the steroid family of nuclear receptors. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Each protein contains distinct functional domains required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to EREs. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are several different isoforms of both ER alpha and ER beta. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; 4; 5; 6; 7*/654 isoform; 7*/819-2 isoform; Er alpha; ER-alpha; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha delta 3*; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor alpha-like; estrogen receptor protein; estrogen receptor, alpha; nuclear receptor; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: BOS_9943; ER; ER-alpha; ERa; ERALPHA; ESR; ESR1; ESRA; Estr; Estra; ESTRR; I79_001166; NR3A1; RNESTROR
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