|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Insect|
|Storage buffer||0.05M Bis-tris propane, pH 9.0, with 0.5M KCl, 50% glycerol, 0.6% CHAPS, 2mM DTT, 275mM Urea|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|Gel Shift (GS)||use as control|
|Western Blot (WB)||use as control|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
RP-311 contains 180 ug of full-length ER Beta in 50 mM bis tris propane (pH 9.0), 500 mM KCl, 50% glycerol, 275 mM Urea, 0.6% w/v CHAPS, and 2 mM DTT.
This recombinant protein was obtained from Baculovirus-infected Sf9 cells and is both hormone binding and estrogen response element binding competent. RP-311 may be used as a control in Western blot experiments with PA1-311 or for gel shift and super shift assays with an appropriate estrogen response element and antibody (PA1-311 can be used for supershift experiments with vitellogenin ERE).
RP-311 calculated molecular weight is 53.4 kDa.
The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Modulation of tumor formation and intestinal cell migration by estrogens in the Apc(Min/+) mouse model of colorectal cancer.
RP-311 was used in western blot to investigate the role of estrogens during tumor formation and intestinal cell migration in the Apc (Min/+) mouse model of colorectal cancer
|Javid SH,Moran AE,Carothers AM,Redston M,Bertagnolli MM||Carcinogenesis (26:587)||2005|