|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:50-1:100|
|Immunohistochemistry (IHC)||See 41 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rabbit , Rat , Cat , Human , Mouse , Chicken Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Clone||1D5 + 6F11|
|Immunogen||Recombinant human estrogen receptor protein|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14104 is a mixture of two monoclonal antibodies that target Estrogen Receptor alpha.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 10mM citrate buffer (pH 6.0) for 10-20 min, followed by cooling at room temperature for 20 min. A recommended positive control is MCF-7 cells.
The Estrogen Receptor alpha (ER alpha) protein is approximately 66kD, though multiple isoforms ranging from 36-66kD have been described. The ER alpha gene (ESR1) consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. Each domain is functionally distinct, required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; Er alpha; ER-alpha; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor protein; Nuclear receptor subfamily 3 group A member 1; nuclear receptor subfamily 3 group A member 2
Gene Aliases: ER; ER-alpha; Era; ESR; ESR1; ESRA; Estr; ESTRR; NR3A1; RNESTROR
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