|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||The region encoding amino acids 1-190 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||1-5 µg/ml|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23214 detects Estrogen Receptor Alpha in Human samples.
MA1-23214 has been successfully used in Immunoprecipitation and Western Blot procedures. Positive control of MCF7 suggested.
MA1-23214 immunogen corresponds to The region encoding amino acids 1-190 expressed in E. coli.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.