Immunofluorescent analysis of Estrogen Receptor Alpha (green) in T47D cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with or without Estrogen Receptor Alpha monoclonal antibody (Product # MA1-310), at a concentration of 10ug/ml overnight at 4C, and then incubated with a Superclonal goat anti-mouse IgG Alexa Fluor 488 secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Avian, Rat, Non-human primate, Bovine, Fish, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.|
|Storage buffer||Dulbecco's PBS with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||10 µg/ml|
|Immunohistochemistry (IHC)||1:20 - 1:100|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-310 detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. MA1-310 detects ER alpha and does not cross react with ER beta.
MA1-310 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, gel shift, flow cytometry, and immunocytochemical experiments. By Western blot, this antibody detects an ~66 kDa protein representing ER alpha from MCF-7 cell extract.
The Estrogen Receptor (ER) is a 66 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tibolone protects astrocytic cells from glucose deprivation through a mechanism involving estrogen receptor beta and the upregulation of neuroglobin expression.
MA1-310 was used in immunocytochemistry to characterize protection of astrocytic cells from glucose deprivation through a mechanism involving estrogen receptor beta and the upregulation of neuroglobin expression by tibolone
|Avila-Rodriguez M,Garcia-Segura LM,Hidalgo-Lanussa O,Baez E,Gonzalez J,Barreto GE||Molecular and cellular endocrinology (433:35)||2016|
Increased gene copy number of VAMP7 disrupts human male urogenital development through altered estrogen action.
MA1-310 was used in immunocytochemistry and western blot to analyze children born with congenital genitourinary tract masculinization disorders by array-comparative genomic hybridization
|Tannour-Louet M,Han S,Louet JF,Zhang B,Romero K,Addai J,Sahin A,Cheung SW,Lamb DJ||Nature medicine (20:715)||2014|
Estrogen modulates the recruitment of myelopoietic cell progenitors in rat through a stromal cell-independent mechanism involving apoptosis.
MA1-310 was used in immunocytochemistry to demonstrate the mechanism for estrogen modulation of the recruitment of myelopoietic cell progenitors in rat
|Shevde NK,Pike JW||Blood (87:2683)||1996|
Immunogold labelling of estradiol receptor in MCF7 cells.
MA1-310 was used in immunocytochemistry to investigate the distribution of estrogen receptor in MCF-7 cells
|Sierralta WD,Bönig I,Thole HH||Cell and tissue research (279:445)||1995|
Establishment of the human BSMZ breast cancer cell line, which overexpresses the erbB-2 and c-myc genes.
MA1-310 was used in immunocytochemistry to establish a human BSMZ breast cancer cell line overexpressing erbB-2 and c-myc
|Watanabe M,Tanaka H,Kamada M,Okano JH,Takahashi H,Uchida K,Iwamura A,Zeniya M,Ohno T||Cancer research (52:5178)||1992|
|Not Applicable||Not Cited||
Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1.
MA1-310 was used in western blot to characterize a feedforward mechanism at the G protein-coupled estrogen receptor 1 that controls estrogen enhaced linkage in the vascular endothelial calmodulin network
|Tran QK,Firkins R,Giles J,Francis S,Matnishian V,Tran P,VerMeer M,Jasurda J,Burgard MA,Gebert-Oberle B||The Journal of biological chemistry (291:10805)||2016|
Inhibition of SHP2 in basal-like and triple-negative breast cells induces basal-to-luminal transition, hormone dependency, and sensitivity to anti-hormone treatment.
MA1-310 was used in western blot to study the role of Src homology phosphotyrosyl phosphatase 2 (SHP2) in triple negative breast cancers
|Zhao H,Agazie YM||BMC cancer (15:null)||2015|
17ß-Estradiol induces supernumerary primordial germ cells in embryos of the polychaete Platynereis dumerilii.
MA1-310 was used in western blot to study the induction of supernumerary primordial germ cells in polychete embryos following 17beta-estradiol treatment
|Lidke AK,Bannister S,Löwer AM,Apel DM,Podleschny M,Kollmann M,Ackermann CF,García-Alonso J,Raible F,Rebscher N||General and comparative endocrinology (196:52)||2014|
Gender matters: estrogen receptor alpha (ER¿) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A).
MA1-310 was used in western blot to study the gender-specific regulation of human UGT1A at the transcriptional level and the mechanistic roles played by ER-alpha and HDAC-1 and -2
|Kalthoff S,Winkler A,Freiberg N,Manns MP,Strassburg CP||Journal of hepatology (59:797)||2013|
Sex steroid receptor evolution and signalling in aquatic invertebrates.
MA1-310 was used in western blot to study the role of sex steroid in reproductive endocrinology of aquatic invertebrates
|Köhler HR,Kloas W,Schirling M,Lutz I,Reye AL,Langen JS,Triebskorn R,Nagel R,Schönfelder G||Ecotoxicology (London, England) (16:131)||2007|
Effects of cadmium on the reproductive axis of Japanese medaka (Oryzias latipes).
MA1-310 was used in western blot to investigate the effect of cadmium on the reproductoin of Japanese medaka
|Tilton SC,Foran CM,Benson WH||Comparative biochemistry and physiology. Toxicology and pharmacology : CBP (136:265)||2003|
Osteoblast differentiation influences androgen and estrogen receptor-alpha and -beta expression.
MA1-310 was used in western blot to investigate the effect of osteoblast differentiation on androgen and estrogen receptor-alpha and -beta expression
|Wiren KM,Chapman Evans A,Zhang XW||The Journal of endocrinology (175:683)||2002|
Transgenerational and developmental exposure of Japanese medaka (Oryzias latipes) to ethinylestradiol results in endocrine and reproductive differences in the response to ethinylestradiol as adults.
MA1-310 was used in western blot to investigate the impact of 17alpha-Ethinylestradiol exposure on Japanese medaka's reproductive and endocrine function in different developmental periods
|Foran CM,Peterson BN,Benson WH||Toxicological sciences : an official journal of the Society of Toxicology (68:389)||2002|
Expression of estrogen receptor alpha and beta in the epiphyseal plate of the rat.
MA1-310 was used in western blot to investigate estrogen receptor alpha and beta expression in the epiphyseal plate of the rat
|van der Eerden BC,Gevers EF,Löwik CW,Karperien M,Wit JM||Bone (30:478)||2002|
Linkage of rapid estrogen action to MAPK activation by ERalpha-Shc association and Shc pathway activation.
MA1-310 was used in western blot to study the mechanism by which E2 rapidly activates MAPK in breast cancer cells
|Song RX,McPherson RA,Adam L,Bao Y,Shupnik M,Kumar R,Santen RJ||Molecular endocrinology (Baltimore, Md.) (16:116)||2002|
Immunohistochemical detection and northern blot analysis of estrogen receptor in osteoblastic cells.
MA1-310 was used in western blot to investigate the expression of estrogen receptor in osteoblastic cells
|Ikegami A,Inoue S,Hosoi T,Mizuno Y,Nakamura T,Ouchi Y,Orimo H||Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (8:1103)||1993|
Highly variable cancer subpopulations that exhibit enhanced transcriptome variability and metastatic fitness.
MA1-310 was used in flow cytometry to characterize enhancement of transcriptome variability and metastatic fitness by highly variable cancer subpopulations
|Nguyen A,Yoshida M,Goodarzi H,Tavazoie SF||Nature communications (7:null)||2016|
Expression of the estrogen receptor in blood neutrophils of dairy cows during the periparturient period.
MA1-310 was used in flow cytometry to investigate the estrogen receptor protein expression level in neutrophils of cows in early lactation.
|Lamote I,Meyer E,De Ketelaere A,Duchateau L,Burvenich C||Theriogenology (65:1082)||2006|
Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
MA1-310 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer
|Kruer TL,Cummins TD,Powell DW,Wittliff JL||Clinical biochemistry (46:1739)||2013|
The negative regulation of the rat aldehyde dehydrogenase 3 gene by glucocorticoids: involvement of a single imperfect palindromic glucocorticoid responsive element.
MA1-310 was used in EMSA assay to study how glucocorticoids affects ALDH3 expression in primary rat hepatocyte cultures and cultured HepG2 cells
|Falkner KC,Xiao GH,Pinaire JA,Pendleton ML,Lindahl R,Prough RA||Molecular pharmacology (55:649)||1999|
Identification of an estrogen response element activated by metabolites of 17beta-estradiol and raloxifene.
MA1-310 was used in EMSA assay to investigate the mechanism for the regulation of gene expression by 17 beta-estradiol and raloxifene
|Yang NN,Venugopalan M,Hardikar S,Glasebrook A||Science (New York, N.Y.) (273:1222)||1996|
Effect of raloxifene and atorvastatin in atherosclerotic process in ovariectomized rats.
MA1-310 was used in immunohistochemistry to study the potential therapeutic effect of combined raloxifene and atorvastatin in atherosclerosis in aged ovariectomized rats
|Cetinkaya Demir B,Uyar Y,Ozbilgin K,Köse C||The journal of obstetrics and gynaecology research (39:229)||2013|
Estradiol inhibits chondrogenic differentiation of mesenchymal stem cells via nonclassic signaling.
MA1-310 was used in immunohistochemistry to study the mechanism by which estradiol inhibits chondrogenic differentiation of mesenchymal stem cells
|Jenei-Lanzl Z,Straub RH,Dienstknecht T,Huber M,Hager M,Grässel S,Kujat R,Angele MK,Nerlich M,Angele P||Arthritis and rheumatism (62:1088)||2010|
Estrous cycle-dependent expression of estrogen receptor alpha in periodontal tissue.
MA1-310 was used in immunohistochemistry to investigate the expression of estrogen receptor alpha in periodontal tissue
|Zhao Q,Tan Z,Guo J,Chen Y||Chronobiology international (24:425)||2007|
Quantitative determination of steroid hormone receptor positive cells in the synovium of patients with rheumatoid arthritis and osteoarthritis: is there a link to inflammation?
MA1-310 was used in immunohistochemistry to quantitate the steroid hormone receptor positive cells in the synovium of rheumatoid arthritic and osteoarthritic patients
|Capellino S,Riepl B,Rauch L,Angele P,Cutolo M,Straub RH||Annals of the rheumatic diseases (66:53)||2007|
Effects of an antiprogestin onapristone on the endometrium of bonnet monkeys: morphometric and ultrastructural studies.
MA1-310 was used in immunohistochemistry to study how the long-term low-dose onapristone treatment affects endometrial development at the subcellular level
|Gopalkrishnan K,Katkam RR,Sachdeva G,Kholkute SD,Padwal V,Puri CP||Biology of reproduction (68:1959)||2003|
Expression of estrogen receptor in the choroidal neovascular membranes in highly myopic eyes.
MA1-310 was used in immunohistochemistry to investigate estrogen receptor expression in choroidal neovascular membranes in myopic eyes
|Kobayashi K,Mandai M,Suzuma I,Kobayashi H,Okinami S||Retina (Philadelphia, Pa.) (22:418)||2002|
Evidence for estrogen receptor expression in germ cell and somatic cell subpopulations in the ovary of the newly hatched chicken.
MA1-310 was used in immunohistochemistry to investigate the expression of estrogen receptor in germ and somatic cell subpoplations in the ovary of newly hatched chicken
|Méndez MC,Chávez B,Echeverría O,Vilchis F,Vázquez Nin GH,Pedernera E||Cell and tissue research (298:145)||1999|
Vascular smooth muscle cells as target for estrogen.
MA1-310 was used in immunohistochemistry to demonstrate the expression of estrogen receptors in vascular smooth muscle cells
|Orimo A,Inoue S,Ikegami A,Hosoi T,Akishita M,Ouchi Y,Muramatsu M,Orimo H||Biochemical and biophysical research communications (195:730)||1993|