Immunofluorescent analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) (Product# MA3-310) shows staining in MCF-7 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha (Product# MA3-310) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Human, Rabbit|
|Published species reactivity||Rabbit|
|Host / Isotype||Mouse|
|Immunogen||Mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) and E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:100 -1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-310 detects estrogen receptor alpha in human and rabbit samples.
MA3-310 has been successfully used in immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects a ~68 kDa protein representing estrogen receptor alpha in MCF-7 cells, human breast tumors, and rabbit vaginal tissue. In immunohistochemistry this antibody recognizes the estrogen receptor alpha in MCF-7 cells, human breast tissue, and human smooth muscle cells.
The MA3-310 immunogen is a mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) and E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits.
MA3-310 was used in immunohistochemistry to examine the effect of estrogen on amoratase expression in the pubococcygeus and bulbospongiosus muscles of female rabbits
|de los Ángeles Carrasco-Ruiz M,García-Villamar V,López-García K,Sánchez-García O,Pacheco P,Cuevas E,Martínez-Gómez M,Castelán F||Cell biochemistry and function (33:188)||2015|
Oestrogen upregulates L-type Ca²¿ channels via oestrogen-receptor- by a regional genomic mechanism in female rabbit hearts.
MA3-310 was used in western blot to investigate the impact of oestrogen and progesterone on calcium signalling in female rabbit hearts
|Yang X,Chen G,Papp R,Defranco DB,Zeng F,Salama G||The Journal of physiology (590:493)||2012|