|Tested species reactivity||Human, Rat|
|Published species reactivity||Sheep|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human ER-alpha (2-180 aa).|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||physiological saline|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
Estrogen Receptors (ER) are members of the steroid family of nuclear receptors. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Each protein contains distinct functional domains required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to EREs. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are several different isoforms of both ER alpha and ER beta. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes.
417000 was used in flow cytometry and immunohistochemistry to study the microscopic and ultrastructure anatomy, secretion composition, and mRNA and protein expression of estrogen receptors a and b and progesterone receptors of the interdigital sinus of cyclic ewes
|Alexandre-Pires G,Martins C,Galvão AM,Correia M,Ramilo D,Quaresma M,Ligeiro D,Nunes T,Caldeira RM,Ferreira-Dias G||Microscopy research and technique (77:313)||2014|