Antibody target was verified by IP Mass Spectrometry to ensure the antibody binds to the antigen stated. View details.
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||15 µg/ml|
|Western Blot (WB)||1:250|
|Tested Species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues R(287) A A N L W P S P L M I K R(300) of the hinge region of human Estrogen Receptor.|
|Storage buffer||PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-25623 detects Estrogen Receptor alpha from human, mouse, rat, and bovine samples.
MA1-25623 has been successfully used in ICC/IF, immunoprecipitation, and Western blot procedures.
The MA1-25623 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues R(287) A A N L W P S P L M I K R(300) of the hinge region of human Estrogen Receptor.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; ER Alpha; ER-alpha; ESR1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: BOS_9943; ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; NR3A1; RNESTROR
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