|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues R(287) A A N L W P S P L M I K R(300) of the hinge region of human Estrogen Receptor.|
|Storage buffer||PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||15 µg/ml|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25623 detects Estrogen Receptor alpha from human, mouse, rat, and bovine samples.
MA1-25623 has been successfully used in ICC/IF, immunoprecipitation, and Western blot procedures.
The MA1-25623 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues R(287) A A N L W P S P L M I K R(300) of the hinge region of human Estrogen Receptor.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
IP-MS enrichment of ESR1 (LFQ intensity): ESR1 was enriched 229-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and ESR1 antibody (Part No. MA1-25623). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.