|Tested species reactivity||Camelid, Human, Non-human primate, Sheep, Pig, Reptile, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide derived from the C terminus of the human estrogen receptor beta 1 isoform, CEDSKSKEGSQNPQSQ.|
|Storage buffer||tissue culture supernatant|
|Contains||0.1% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/5-1/50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In immunohistochemical studies, this antibody has shown positive nuclear staining in cells within the ovary, uterus, oviduct, breast, testis, prostate, bladder and male reproductive duct. There are also reports of cell staining in the adenohypophysis, demonstrating cytoplasmic expression.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) or 0.05M glycine/EDTA (pH 8) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is ovary.
The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ERß expression and breast cancer risk prediction for women with atypias.
MA1-81281 was used in immunohistochemistry - paraffin section to assess ERbeta expression in breast cancer tissues
|Hieken TJ,Carter JM,Hawse JR,Hoskin TL,Bois M,Frost M,Hartmann LC,Radisky DC,Visscher DW,Degnim AC||Cancer prevention research (Philadelphia, Pa.) (8:1084)||2015|
The Estrogen Pathway: Estrogen Receptor-¿, Progesterone Receptor, and Estrogen Receptor-ß Expression in Radical Cystectomy Urothelial Cell Carcinoma Specimens.
MA1-81281 was used in immunohistochemistry to discuss the role of the estrogen pathway in bladder cancer development.
|Tan W,Boorjian S,Advani P,Farmer S,Lohse C,Cheville J,Kwon E,Leibovich B||Clinical genitourinary cancer (13:476)||2015|
Development, characterization, and applications of a novel estrogen receptor beta monoclonal antibody.
MA1-81281 was used in immunohistochemistry to develop and characterize a new ERbeta monoclonal antibody in human cells
|Wu X,Subramaniam M,Negron V,Cicek M,Reynolds C,Lingle WL,Goetz MP,Ingle JN,Spelsberg TC,Hawse JR||Journal of cellular biochemistry (113:711)||2012|
Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle.
MA1-81281 was used in western blot to test the effect of estrogen on cigarette smoke-induced airway dysfunction.
|Sathish V,Freeman MR,Long E,Thompson MA,Pabelick CM,Prakash YS||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (36:1101)||2015|
ERß1: characterization, prognosis, and evaluation of treatment strategies in ER¿-positive and -negative breast cancer.
MA1-81281 was used in immunohistochemistry - paraffin section to characterize ER-beta1 as a treatment strategy for prognosis and evaluztion in ER-alpha positive and negative breast cancer
|Reese JM,Suman VJ,Subramaniam M,Wu X,Negron V,Gingery A,Pitel KS,Shah SS,Cunliffe HE,McCullough AE,Pockaj BA,Couch FJ,Olson JE,Reynolds C,Lingle WL,Spelsberg TC,Goetz MP,Ingle JN,Hawse JR||BMC cancer (14:null)||2014|
Invasive ductular carcinoma in 2 rhesus macaques (Macaca mulatta).
MA1-81281 was used in immunohistochemistry - paraffin section to assess the prognostic value of immunohistochemical markers in nonhuman primates
|Beck AP,Brooks A,Zeiss CJ||Comparative medicine (64:314)||2014|