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Immunofluorescent analysis of Estrogen Related Receptor beta (green) in NRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a Estrogen Related Receptor beta polyclonal antibody (Product # PA1-315) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-315 detects ERR2 from mouse and rat kidney extracts.
PA1-315 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~48 kDa protein representing ERR2 from rat kidney extract.
The PA1-315 immunogen is a synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2. This peptide (Cat. # PEP-251) is available for use in neutralization and control experiments.
Estrogen-related receptor 2 (ERR beta, ERR2) is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members share an almost identical DNA-binding domain, which has 68% amino acid identity with that of estrogen receptor. It has been shown the ERR2 plays a critical functional role in placental development.
The corresponding gene for the ERR2 is NR3B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ERR-beta; estrogen receptor related 2; Estrogen receptor-like 2; Estrogen-related receptor beta; estrogen-related receptor, beta; Nuclear receptor subfamily 3 group B member 2; Steroid hormone receptor ERR2
Err-2; Err2; Errb; Esrl2; Esrrb; Estrrb; Nr3b2