Immunofluorescent analysis of Estrogen Related Receptor beta (green) in NRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a Estrogen Related Receptor beta polyclonal antibody (Product # PA1-315) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-315 detects ERR2 from mouse and rat kidney extracts.
PA1-315 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~48 kDa protein representing ERR2 from rat kidney extract.
The PA1-315 immunogen is a synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2. This peptide (Cat. # PEP-251) is available for use in neutralization and control experiments.
Estrogen-related receptor 2 (ERR beta, ERR2) is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members share an almost identical DNA-binding domain, which has 68% amino acid identity with that of estrogen receptor. It has been shown the ERR2 plays a critical functional role in placental development.
The corresponding gene for the ERR2 is NR3B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.