|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Jurkat and HeLa (human cell lines)|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 2 publications below|
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles.
10628D was used in western blot to examine the influence of xeno-free expansion media on biological properties of umbilical cord mesenchymal stem cells and their extracellular vesicles
|Bobis-Wozowicz S,Kmiotek K,Kania K,Karnas E,Labedz-Maslowska A,Sekula M,Kedracka-Krok S,Kolcz J,Boruczkowski D,Madeja Z,Zuba-Surma EK||Journal of molecular medicine (Berlin, Germany) (95:205)||2017|
Microvesicle Cargo and Function Changes upon Induction of Cellular Transformation.
10628D was used in western blot to report that oncogenic transformation results in microvesicles
|Kreger BT,Dougherty AL,Greene KS,Cerione RA,Antonyak MA||The Journal of biological chemistry (291:19774)||2016|