Immunofluorescent analysis of Phalloidin (magenta) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
|Tested species reactivity||Bovine, Hamster, Human, Marsupial, Mouse, Non-human primate, Rat|
|Published species reactivity||Dog, Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A recombinant fragment of human ezrin corresponding to aa 362-585|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13859 targets Ezrin in IHC (P), IP, ICC/IF and WB applications and shows reactivity with Bovine, Hamster, Human, Marsupial, mouse, Non-human primate, and Rat samples.
The MA5-13859 immunogen is a recombinant fragment of human ezrin corresponding to aa 362-585.
Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma.
MA5-13859 was used in immunohistochemistry to study the prognostic value of fascin-1, ezrin and paxillin expression in laryngeal squamous cell carcinoma
|Gao W,Zhang C,Feng Y,Chen G,Wen S,Huangfu H,Wang B||PloS one (7:null)||2012|
Expression of ezrin in human embryonic, fetal, and normal adult tissues.
MA5-13859 was used in immunohistochemistry and western blot to study the immunohistochemical expression of ezrin in human embryonic, fetal, and normal adult tissues
|Xie JJ,Zhang FR,Tao LH,Lü Z,Xu XE,Xu LY,Li EM||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (59:1001)||2011|
Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma.
MA5-13859 was used in immunohistochemistry to investigate the expression and the prognostic significance of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma
|Wang YY,Chen WL,Huang ZQ,Yang ZH,Zhang B,Wang JG,Li HG,Li JS||Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (112:96)||2011|
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
MA5-13859 was used in immunohistochemistry to investigate the diagnostic value of Ezrin and E-cadherin in esophageal squamous cell carcinoma
|Zhai JW,Yang XG,Yang FS,Hu JG,Hua WX||Chinese journal of cancer (29:317)||2010|
Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma.
MA5-13859 was used in immunohistochemistry to study the role of the NF2 tumor suppressor Merlin and EGFR in the tumorigenicity of renal cell carcinoma using a novel murine model
|Morris ZS,McClatchey AI||Proceedings of the National Academy of Sciences of the United States of America (106:9767)||2009|
A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays.
MA5-13859 was used in immunohistochemistry to identify prognostic signatures in breast carcinomas using quantitative immunocytochemical assays
|Charpin C,Secq V,Giusiano S,Carpentier S,Andrac L,Lavaut MN,Allasia C,Bonnier P,Garcia S||International journal of cancer (124:2124)||2009|
Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
MA5-13859 was used in immunohistochemistry to investigate the influence of ezrin-radixin-moesin-binding phosphoprotein on biliary epithelial cell proliferation
|Fouassier L,Rosenberg P,Mergey M,Saubaméa B,Clapéron A,Kinnman N,Chignard N,Jacobsson-Ekman G,Strandvik B,Rey C,Barbu V,Hultcrantz R,Housset C||The American journal of pathology (174:869)||2009|
Adhesion molecules as prognostic markers in pancreatic adenocarcinoma.
MA5-13859 was used in immunohistochemistry to study the prognostic value of ezrin, radixin, moesin and E-cadherin in pancreatic ductal adenocarcinoma
|Torer N,Kayaselcuk F,Nursal TZ,Yildirim S,Tarim A,Nòyan T,Karakayali H||Journal of surgical oncology (96:419)||2007|
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
MA5-13859 was used in immunohistochemistry to study the relationship between ezrin expression and clinicopathological parameters in intestinal and diffuse gastric carcinoma
|Bal N,Yildirim S,Nursal TZ,Bolat F,Kayaselcuk F||World journal of gastroenterology (13:3726)||2007|
Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
MA5-13859 was used in immunohistochemistry to examine the prognostic significance of cytoplasmic ezrin expression for head and neck squamous cell carcinoma
|Madan R,Brandwein-Gensler M,Schlecht NF,Elias K,Gorbovitsky E,Belbin TJ,Mahmood R,Breining D,Qian H,Childs G,Locker J,Smith R,Haigentz M,Gunn-Moore F,Prystowsky MB||Head and neck (28:1018)||2006|
Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma.
MA5-13859 was used in immunohistochemistry to examine the prognostic significance of ezrin in osteosarcoma
|Park HR,Jung WW,Bacchini P,Bertoni F,Kim YW,Park YK||Pathology, research and practice (202:509)||2006|
Ezrin expression in prostate cancer and benign prostatic tissue.
MA5-13859 was used in immunohistochemistry to study the expression of the ezrin in benign and cancerous prostatic tissue
|Valdman A,Fang X,Pang ST,Nilsson B,Ekman P,Egevad L||European urology (48:852)||2005|
Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas.
MA5-13859 was used in immunohistochemistry to study the prognostic significance of zrin in highly malignant soft tissue sarcomas
|Weng WH,Ahlén J,Aström K,Lui WO,Larsson C||Clinical cancer research : an official journal of the American Association for Cancer Research (11:6198)||2005|
Stromal cells in hemangioblastoma: neuroectodermal differentiation and morphological similarities to ependymoma.
MA5-13859 was used in immunohistochemistry to study stromal cells in hemangioblastoma
|Ishizawa K,Komori T,Hirose T||Pathology international (55:377)||2005|
Myocilin is expressed in the glomerulus of the kidney and induced in mesangioproliferative glomerulonephritis.
MA5-13859 was used in immunohistochemistry to study the role of myocilin in mesangioproliferative glomerulonephritis
|Goldwich A,Baulmann DC,Ohlmann A,Flügel-Koch C,Schöcklmann H,Tamm ER||Kidney international (67:140)||2005|
Expression of ezrin in prostatic intraepithelial neoplasia.
MA5-13859 was used in immunohistochemistry to study ezrin expression in prostatic intraepithelial neoplasia
|Pang ST,Fang X,Valdman A,Norstedt G,Pousette A,Egevad L,Ekman P||Urology (63:609)||2004|
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
MA5-13859 was used in immunohistochemistry to investigate the association between ezrin immunoreactivity and survival in ovarian carcinoma patients
|Moilanen J,Lassus H,Leminen A,Vaheri A,Bützow R,Carpén O||Gynecologic oncology (90:273)||2003|
Gene expression profiling of androgen deficiency predicts a pathway of prostate apoptosis that involves genes related to oxidative stress.
MA5-13859 was used in immunohistochemistry to investigate the alteration of gene expression induced by androgen deficiency in rat prostate
|Pang ST,Dillner K,Wu X,Pousette A,Norstedt G,Flores-Morales A||Endocrinology (143:4897)||2002|
Thyroid hormone-deficient period prior to the onset of hearing is associated with reduced levels of beta-tectorin protein in the tectorial membrane: implication for hearing loss.
MA5-13859 was used in immunohistochemistry to investigate the role of tectorins in the development of auditory system
|Knipper M,Richardson G,Mack A,Müller M,Goodyear R,Limberger A,Rohbock K,Köpschall I,Zenner HP,Zimmermann U||The Journal of biological chemistry (276:39046)||2001|
A-kinase anchoring proteins regulate compartmentalized cAMP signaling in airway smooth muscle.
MA5-13859 was used in western blot to study the role of A-kinase anchor proteins in regulating airway smooth muscle beta2-adrenergic receptor signaling
|Horvat SJ,Deshpande DA,Yan H,Panettieri RA,Codina J,DuBose TD,Xin W,Rich TC,Penn RB||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:3670)||2012|
The effect of radixin knockdown on the expression and efflux function of MRP2 in SGC-7901 cells.
MA5-13859 was used in western blot to study MRP2 expression and function in human gastric carcinoma cells following knockdown of radixin
|He XJ,Wang WR,Zhang Y,Yang Q||European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (46:426)||2012|
Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins.
MA5-13859 was used in western blot to study the roles of STIM1 and Orai1 in the mechanism by which streptolysin O alters epithelial cell Ca(2+) signaling and the significance for Streptococcus pyogenes respiratory infections
|Usmani SM,von Einem J,Frick M,Miklavc P,Mayenburg M,Husmann M,Dietl P,Wittekindt OH||Cellular microbiology (14:299)||2012|
Activation of Ras requires the ERM-dependent link of actin to the plasma membrane.
MA5-13859 was used in western blot to study the role of ezrin/radixin/moesin proteins and actin in the activation of Ras
|Sperka T,Geissler KJ,Merkel U,Scholl I,Rubio I,Herrlich P,Morrison HL||PloS one (6:null)||2011|
The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain.
MA5-13859 was used in western blot to identify the relationship between sphingomyelin synthetase 2 levels and drug transporter expression
|Zhang Y,Dong J,Zhu X,Wang W,Yang Q||Biochemical pharmacology (82:287)||2011|
ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells.
MA5-13859 was used in western blot to study the role of ERM proteins in cancer cell metastasis and tumor progression
|Ou-Yang M,Liu HR,Zhang Y,Zhu X,Yang Q||Biochimie (93:954)||2011|
Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice.
MA5-13859 was used in western blot to study the expression and role of ezrin in thymocytes and T cell development in mice
|Shaffer MH,Huang Y,Corbo E,Wu GF,Velez M,Choi JK,Saotome I,Cannon JL,McClatchey AI,Sperling AI,Maltzman JS,Oliver PM,Bhandoola A,Laufer TM,Burkhardt JK||PloS one (5:null)||2010|
p35 is required for CDK5 activation in cellular senescence.
MA5-13859 was used in western blot to study the role of p35 in CDK5 activation in cellular senescence
|Mao D,Hinds PW||The Journal of biological chemistry (285:14671)||2010|
Ezrin mediates c-Myc actions in prostate cancer cell invasion.
MA5-13859 was used in western blot to investigate the role of c-Myc in the development of invasive tumors
|Chuan YC,Iglesias-Gato D,Fernandez-Perez L,Cedazo-Minguez A,Pang ST,Norstedt G,Pousette A,Flores-Morales A||Oncogene (29:1531)||2010|
Involvement of CD44v6 in InlB-dependent Listeria invasion.
MA5-13859 was used in western blot to examine the role of CD44v6 in the infection of Listeria monocytogenes
|Jung C,Matzke A,Niemann HH,Schwerk C,Tenenbaum T,Orian-Rousseau V||Molecular microbiology (72:1196)||2009|
A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.
MA5-13859 was used in western blot to study the mechanism for the apical delivery of podocalyxin/Gp135
|Yu CY,Chen JY,Lin YY,Shen KF,Lin WL,Chien CL,ter Beest MB,Jou TS||Molecular biology of the cell (18:1710)||2007|
Androgen induction of prostate cancer cell invasion is mediated by ezrin.
MA5-13859 was used in western blot to investigate the mechanism for prostate cancer cell invasion induced by androgen
|Chuan YC,Pang ST,Cedazo-Minguez A,Norstedt G,Pousette A,Flores-Morales A||The Journal of biological chemistry (281:29938)||2006|
Slit diaphragms contain tight junction proteins.
MA5-13859 was used in immunoprecipitation to study the presence of tight junction proteins in slit diaphragms
|Fukasawa H,Bornheimer S,Kudlicka K,Farquhar MG||Journal of the American Society of Nephrology : JASN (20:1491)||2009|