|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminus of the 69 kDa isoform of the hCdc4 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4253 detects the F-box hCdc4 (69 kDa isoform) protein in human samples.
PA1-4253 has been successfully used in Western blot procedures.
PA1-4253 immunizing peptide corresponds to the N terminus of the 69 kDa isoform of the hCdc4 protein.
Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid freeze/thaw cycles.
Cyclin E, one of the activators of the cyclin-dependent kinase Cdk2, is critical for the initiation of DNA replication and other S-phase functions. The proper timing and amplitude of cyclin E expression seem to be important, because elevated levels of cyclin E have been associated with a variety of malignancies and constitutive expression of cyclin E leads to genomic instability. Phosphorylated cyclin E depends on the novel human gene, hCdc4, that was found to be mutated in a cell line derived from breast cancer that expressed extremely high levels of cyclin E.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.