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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminus of the 69 kDa isoform of the hCdc4 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4253 detects the F-box hCdc4 (69 kDa isoform) protein in human samples.
PA1-4253 has been successfully used in Western blot procedures.
PA1-4253 immunizing peptide corresponds to the N terminus of the 69 kDa isoform of the hCdc4 protein.
Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid freeze/thaw cycles.
Cyclin E, one of the activators of the cyclin-dependent kinase Cdk2, is critical for the initiation of DNA replication and other S-phase functions. The proper timing and amplitude of cyclin E expression seem to be important, because elevated levels of cyclin E have been associated with a variety of malignancies and constitutive expression of cyclin E leads to genomic instability. Phosphorylated cyclin E depends on the novel human gene, hCdc4, that was found to be mutated in a cell line derived from breast cancer that expressed extremely high levels of cyclin E.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AGO; archipelago; CDC4; DKFZp686F23254; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila); F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; FBW6; FBW7; FBX30; FBXO30; FBXW6; FLJ16457; homolog of C elegans sel-10; SEL10
AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; FBXW7; hAgo; hCdc4; SEL-10; SEL10