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|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Thioglycollate stimulated peritoneal macrophages from C57/BL mice.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hrs. Citrate buffer (pH 6.0) is recommended for tissues fixed for more than 24 hrs. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Mouse EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) or cell surface glycoprotein F4/80 is a cell surface glycoprotein and a member of the EGF TM7 family of proteins. It shares 68% overall amino acid identity with human EGF-module-containing-mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cell surface glycoprotein F4/80; DD7A5-7, EGF-TM7, F4/80, Gpf480, Ly71, TM7LN3,EGF-like module receptor 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71
Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3