Invitrogen

FAAH Monoclonal Antibody (J.896.7)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (17) FAAH antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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FAAH Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of FAAH was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FAAH Monoclonal Antibody (J.896.7) (Product # MA5-14808) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents cells with no primary antibody to assess background. Panel f shows PC-3 cells with low expression of FAAH. The images were captured at 60X magnification..

FAAH Antibody in Western Blot (WB) — Western blot analysis of FAAH1 in extracts from human brain using FAAH1 monoclonal antibody (Product # MA5-14808).

FAAH Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Higher expression of FAAH was observed in LNCaP cells in comparison to PC-3 cells using FAAH Monoclonal Antibody (J.896.7) (Product # MA5-14808) in immunocytochemistry. (doi: 10.1002/ijc.23674). {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunoprecipitation (IP)

1:50

Western Blot (WB)

1:1000

Product Specifications

Species

Human, Mouse

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

J.896.7

Immunogen

Synthetic peptide corresponding to the sequence of human FAAH1

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_11006482

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Degrades bioactive fatty acid amides like oleamide, the endogenous levels of cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: amide hydrolase; Anandamide amidohydrolase 1; FA2H; fatty acid amide hydrolase protein; Fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase; Oleamide hydrolase 1

Gene Aliases: AW412498; FAAH; FAAH-1; FAAH1; PSAB

UniProt ID: (Human) O00519, (Mouse) O08914

Entrez Gene ID: (Human) 2166, (Mouse) 14073

Molecular Function: hydrolase ligase transferase