|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length, human FADD protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20168 detects FADD in human samples.
MA1-20168 has been successfully used in ELISA and immunoprecipitation procedures.
The MA1-20168 immunogen is recombinant, full length, human FADD protein.
FADD (Fas Associated Death Domain) is an apoptosis adapter molecule enabling transduction of the apoptosis signal initiated via the FasL/Fas receptor interaction. The protein contains a C-terminal death domain that interacts with the Fas receptor death domain. The N-terminus contains a death effectors domain (DED, which recruits caspase to the death inducing signaling complex (DISC) and initiates the apoptotic caspase cascade. FADD is implicated in non-apoptotic cellular pathways such as the regulation of cell cycle machinery in T lymphocytes. This is connected to the phosphorylation state of FADD and to the FasL/TRAIL-induced transcriptional activation of c-fos protooncogene. FADD also interacts with the hepatitis C virus core protein in the HEK-293 cell line.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.