|Tested species reactivity||Amphibian, Dog, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16676 targets FAK-Focal Adhesion Kinase in IF and WB applications and shows reactivity with Amphibian, Canine, Human, mouse, and Rat samples.
The PA5-16676 immunogen is recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK.
FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration.
PA5-16676 was used in western blot to study the modulation of cell polarity and migration and the roles played by VANGL2 and MMP14
|Williams BB,Cantrell VA,Mundell NA,Bennett AC,Quick RE,Jessen JR||Journal of cell science (125:2141)||2012|
Osteonectin downregulates E-cadherin, induces osteopontin and focal adhesion kinase activity stimulating an invasive melanoma phenotype.
PA5-16676 was used in western blot to investigate the consequences of osteonectin expression in melanoma cells
|Smit DJ,Gardiner BB,Sturm RA||International journal of cancer (121:2653)||2007|
Inducible changes in cell size and attachment area due to expression of a mutant SWI/SNF chromatin remodeling enzyme.
PA5-16676 was used in western blot to investigate the effect of expression of mutant SWI/SNF on cell shape and cell attachment
|Hill DA,Chiosea S,Jamaluddin S,Roy K,Fischer AH,Boyd DD,Nickerson JA,Imbalzano AN||Journal of cell science (117:5847)||2004|
The NH2-terminal region of focal adhesion kinase reconstitutes high affinity IgE receptor-induced secretion in mast cells.
PA5-16676 was used in western blot to investigate the role of the NH2-terminal region of focal adhesion kinase FAK in the affinity IgE receptor-induced secretion in mast cells
|Vial D,Okazaki H,Siraganian RP||The Journal of biological chemistry (275:28269)||2000|
Culture-induced increase in alpha integrin subunit expression in retinal pigment epithelium is important for improved resurfacing of aged human Bruch's membrane.
PA5-16676 was used in immunocytochemistry to investigate the role of integrins iun the adhesion of retinal pigment epithelium on aged human Bruch membrane
|Gullapalli VK,Sugino IK,Zarbin MA||Experimental eye research (86:189)||2008|