Immunofluorescent analysis of FAK2 in A549 cells using a FAK2 recombinant rabbit monoclonal antibody (Product # 700183) at a dilution of 20ug/ml in the absence of peptide (top) or in the presence of the immunogenic peptide (bottom) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000 and actin staining using Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (left), AF488 signal only (middle) and composite image with Phalloidin (right).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 780-880 of Q14289.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 ul|
|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||4-6 ug/ml|
|Western Blot (WB)||1-3µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chimpanzee, equine, mouse, orangutan, rat and Rhesus monkey based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PyK2, also known as protein tyrosine kinase, is known to be expressed in the central nervous system and from cells derived from hematopoietic lineages. In response to various stimuli that increase the intracellular calcium concentration, PyK2 gets rapidly phosphorylated on the tyrosine residues. On activation of the Pyk2 protein, the MAP kinase signaling pathway is activated and its activation brings about modulation of the ion channel function. The phosphorylation of tyrosin is brought about by reactions involving protein tyrosine phosphatases and the RAFT/Pyk2 kinase proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.