Immunofluorescent analysis of FANCJ showing staining in the cytoplasm of 293T cells. 293T cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a FANCJ polyclonal antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 52 and 291 of Human FANCJ|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27096 targets FANCJ in IF and WB applications and shows reactivity with Human samples.
The PA5-27096 immunogen is recombinant fragment corresponding to a region within amino acids 52 and 291 of Human FANCJ.
The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations.
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