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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Fas/CD95 cDNA.|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µl for 10^6 cells or 1 µg/100 µl blood|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
MA1-20163 detects Fas in human samples.
MA1-20163 has been successfully used in FACS and Western blot procedures.
The MA1-20163 immunogen corresponds to human Fas/CD95 cDNA which was transfected into mouse L cells.
Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. One of the most studied receptors include the Fas receptor. Apoptosis mediated by both signaling cascades results in activation of a family of cystein proteases known as caspases. Engagement of Fas by its ligand, or by an appropriate antibody, results in the rapid induction of programmed cell death in susceptible cell lines. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Early induction of polyfunctional simian immunodeficiency virus (SIV)-specific T lymphocytes and rapid disappearance of SIV from lymph nodes of sooty mangabeys during primary infection.
MA1-20163 was used in flow cytometry to study the contribution of the cellular immune response in maintaining nonpathogenic SIV infection in sooty mangabeys.
|Meythaler M,Wang Z,Martinot A,Pryputniewicz S,Kasheta M,McClure HM,O'Neil SP,Kaur A||Journal of immunology (Baltimore, Md. : 1950) (186:5151)||2011|
Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase.
MA1-20163 was used in blocking assay to study the intracellular signaling generated after cross-linking of CD95 and apoptosis.
|Cifone MG,De Maria R,Roncaioli P,Rippo MR,Azuma M,Lanier LL,Santoni A,Testi R||The Journal of experimental medicine (180:1547)||1994|
ALPS1A; APO-1; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily, member 6
ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6