Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE).<br />Histograms:<br />Red – Isotype mouse IgG1 control staining<br />Black – standard anti-CD95 monoclonal antibody (DX2)<br />Green – anti-CD95 (LT95)<br /><br />A – JURKAT human peripheral blood T cell leukemia cell line<br />B – RAMOS human Burkitt lymphoma cell line<br />C – CEM human leukemia cell line<br />D – MOLT-4 human acute lymphoblastic T cell leukemia cell line
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||HUT-78 human T cell lymphoma cell line|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 ul/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
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