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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues 157-343 of human Fas Ligand.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:100 to 1:1000|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:10 to 1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100 to 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-4804 detects Fas Ligand (FasL, CD95L) from human samples.
MA1-4804 has been successfully used in immunohistochemistry (paraffin and frozen), immunofluorescence, immunoprecipitation and Western blot procedures. Negative after pre-adsorption with FasL-peptide for Western blot and paraffin sections. By Western blot, this antibody detects a ~45 kDa protein representing the membrane bound form of FasL in human T-lymphocyte, NK, testis, eye, thymus, liver, ovary, lung, heart, and kidney samples.
The MA1-4804 immunogen is a synthetic peptide corresponding to residues 157-343 of human Fas Ligand.
Fas ligand (FasL, CD 95L) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas (CD 95, Apo-1) is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family.
As a member of the TNF-cytokine family FasL induces apoptosis when interacting with its receptor Fas. FasL may exist as either membrane bound (45 kD) or soluble forms (26 kD).
The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of FasL to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins.
FasL is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. FasL expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney.
The Fas/FasL system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer. It is assumed that induction of apoptosis through FasL is predominantly involved in anti-viral immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
APO1; apoptosis (APO-1) antigen ligand 1; apoptosis antigen ligand; apoptosis antigen ligand 1; CD178; CD95 ligand; CD95L; fas antigen ligand; Fas Ligand; Fas ligand (TNF superfamily, member 6); Fas-LG; FasL; mutant tumor necrosis factor family member 6; tumor necrosis factor ligand 1A
ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; FASLG; TNFSF6; TNLG1A