Invitrogen

FASN Monoclonal Antibody (C.60.10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 References

View all (34) FASN antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (7)
Advanced Verification (2)

FIGURE: 1 / 9

FASN Antibody (MA5-14887) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK 293 (Lane 2), PANC-1 (Lane 3), Caco-2 (Lane 4), A549 (Lane 5), MCF7 (Lane 6), SK-BR-3 (Lane 7), tissue extracts of Mouse Liver (Lane 8), Mouse Adipose (Lane 9) and Mouse Brown Adipose (Lane 10). The blot was probed with Anti-FASN Monoclonal Antibody (Product # MA5-14887, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 diluti... View More

Product Image Gallery

FASN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Fatty Acid Synthase in paraffin-embedded breast carcinoma using a Fatty Acid Synthase monoclonal antibody (Product # MA5-14887).

FASN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with FASN siRNA and reduction of signal was observed in Western Blot using FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887). {KD}

Product Details

MA5-14887

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.60.10

Immunogen

Synthetic peptide around Gly46 corresponding to the sequence of human fatty acid synthase

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

5 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10980075

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Naringin attenuates fructose-induced NAFLD progression in rats through reducing endogenous triglyceride synthesis and activating the Nrf2/HO-1 pathway.

Frontiers in pharmacology

Pengnet S,Sumarithum P,Phongnu N,Prommaouan S,Kantip N,Phoungpetchara I,Malakul W

MA5-14887 was used in Western Blotting to emonstrate that oral administration of naringin protects against fructose-induced hepatic steatosis by decreasing DNL and TG synthesis and that naringin could prevent NAFLD progression via targeting the Nrf2/HO-1 and the NF-κB/TNF-α pathways.

Tue Jan 03 00:00:00 EST 2023

Extra-mitochondrial mouse frataxin and its implications for mouse models of Friedreich's ataxia.

Scientific reports

Weng L,Laboureur L,Wang Q,Guo L,Xu P,Gottlieb L,Lynch DR,Mesaros C,Blair IA

MA5-14887 was used in Western Blotting to demonstrate the role of extra-mitochondrial frataxin in the etiology of Friedreich's ataxia.

Fri Sep 25 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Acyl-[acyl-carrier-protein] hydrolase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase

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Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

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UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

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Entrez Gene ID: (Human) 2194, (Mouse) 14104, (Rat) 50671

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Function(s)

osteoblast differentiation acetyl-CoA metabolic process fatty acid metabolic process fatty acid biosynthetic process pantothenate metabolic process mammary gland development positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process oxidation-reduction process cellular response to interleukin-4 cell-cell adhesion lipid metabolic process metabolic process biosynthetic process lipid biosynthetic process

Process(es)

Performance Guarantee

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FASN Monoclonal Antibody (C.60.10)

FASN Antibody (MA5-14887) in WB

Product Details

MA5-14887

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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