Invitrogen

FASN Monoclonal Antibody (C.60.10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (31) FASN antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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FASN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

FASN Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Fatty Acid Synthase in paraffin-embedded breast carcinoma using a Fatty Acid Synthase monoclonal antibody (Product # MA5-14887).

FASN Antibody in Western Blot (WB) — Knockdown of FASN was achieved by transfecting MCF7 with FASN specific siRNAs (Silencer® select Product # s5030). Western blot analysis (Fig. a) was performed using whole cell extracts from the FASN knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FASN.

FASN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with FASN siRNA and reduction of signal was observed in Western Blot using FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887). {KD}

Product Details

MA5-14887

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunohistochemistry (Frozen) (IHC (F))	1:50	-
Immunocytochemistry (ICC/IF)	1:50	-
Immunoprecipitation (IP)	1:50	-

Product Specifications
Species Reactivity	Bovine, Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Monoclonal
Type	Antibody
Clone	C.60.10
Immunogen	Synthetic peptide around Gly46 corresponding to the sequence of human fatty acid synthase
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	<0.02% sodium azide
Storage conditions	-20°C
RRID	AB_10980075

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extra-mitochondrial mouse frataxin and its implications for mouse models of Friedreich's ataxia.

Scientific reports

Weng L,Laboureur L,Wang Q,Guo L,Xu P,Gottlieb L,Lynch DR,Mesaros C,Blair IA

MA5-14887 was used in Western Blotting to demonstrate the role of extra-mitochondrial frataxin in the etiology of Friedreich's ataxia.

Fri Sep 25 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Acyl-[acyl-carrier-protein] hydrolase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase

Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

UniProt ID: (Bovine) Q71SP7, (Human) P49327, (Mouse) P19096, (Rat) P12785

Entrez Gene ID: (Bovine) 281152, (Human) 2194, (Mouse) 14104, (Rat) 50671

Function(s)