Invitrogen

FASN Monoclonal Antibody (C.60.10)

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

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FASN Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

FASN Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Fatty Acid Synthase in HeLa cells using a Fatty Acid Synthase monoclonal antibody (Product # MA5-14887) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

FASN Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Fatty Acid Synthase in paraffin-embedded breast carcinoma using a Fatty Acid Synthase monoclonal antibody (Product # MA5-14887).

FASN Antibody in Western Blot (WB) — Knockdown of FASN was achieved by transfecting MCF7 with FASN specific siRNAs (Silencer® select Product # s5030). Western blot analysis (Fig. a) was performed using whole cell extracts from the FASN knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FASN.

FASN Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK 293 (Lane 2), PANC-1 (Lane 3), Caco-2 (Lane 4), A549 (Lane 5), MCF7 (Lane 6), SK-BR-3 (Lane 7), tissue extracts of Mouse Liver (Lane 8), Mouse Adipose (Lane 9) and Mouse Brown Adipose (Lane 10). The blot was probed with Anti-FASN Monoclonal Antibody (Product # MA5-14887, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 273 kDa band corresponding to FASN was observed across the cell lines and tissues tested.

FASN Antibody in Western Blot (WB) — Western blot analysis of Fatty Acid Synthase in extracts from 293 and Hela cells using Fatty Acid Synthase monoclonal antibody (Product # MA5-14887).

FASN Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with FASN siRNA and reduction of signal was observed in Western Blot using FASN Monoclonal Antibody (C.60.10) (Product # MA5-14887). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50

-

Immunofluorescence (IF)

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunoprecipitation (IP)

1:50

-

Western Blot (WB)

1:1000

-

Product Specifications

Species

Bovine, Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.60.10

Immunogen

Synthetic peptide around Gly46 corresponding to the sequence of human fatty acid synthase

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10980075

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; Oleoyl-[acyl-carrier-protein] hydrolase; short chain dehydrogenase/reductase family 27X, member 1

Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

Entrez Gene ID: (Bovine) 281152, (Human) 2194, (Mouse) 14104, (Rat) 50671

Molecular Function: acyltransferase dehydrogenase esterase hydrolase ligase methyltransferase oxidoreductase transferase

fatty acid biosynthetic process oxidation-reduction process osteoblast differentiation acetyl-CoA metabolic process fatty acid metabolic process pantothenate metabolic process mammary gland development positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process cellular response to interleukin-4 cell-cell adhesion lipid metabolic process metabolic process biosynthetic process lipid biosynthetic process

Process(es)

[acyl-carrier-protein] S-acetyltransferase activity [acyl-carrier-protein] S-malonyltransferase activity 3-oxoacyl-[acyl-carrier-protein] synthase activity 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity oleoyl-[acyl-carrier-protein] hydrolase activity (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity myristoyl-[acyl-carrier-protein] hydrolase activity palmitoyl-[acyl-carrier-protein] hydrolase activity phosphopantetheine binding enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity 3-oxo-glutaryl-[acp] methyl ester reductase activity 3-oxo-pimeloyl-[acp] methyl ester reductase activity fatty acid synthase activity enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity protein binding drug binding protein homodimerization activity poly(A) RNA binding NADPH binding cadherin binding involved in cell-cell adhesion catalytic activity zinc ion binding acyl-[acyl-carrier-protein] hydrolase activity oxidoreductase activity transferase activity hydrolase activity hydrolase activity, acting on ester bonds lyase activity 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity identical protein binding